Comparison of plasmids in strains of Zymomonas mobilis

H. W. Stokes, E. L. Dally, M. D. Yablonsky, D. E. Eveleigh*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)

Abstract

Four strains of Zymomonas mobilis were examined for their resistance to antimicrobial agents and found to have similar resistance profiles. Plasmid DNA was extracted and purified by CsCl dye-buoyant density centrifugation; molecular weights were determined by agarose gel electrophoresis and electron microscopy. All four strains harbored a large plasmid (46 × 106 Da) and a smaller plasmid (16-21 × 106 Da) whose molecular weight was strain dependent. Two strains, Ag11 and ATCC 10988, had smaller plasmids of unique molecular weight. Homology existed between the plasmids in the four strains as shown by cross-reaction in DNA-DNA blot hybridizations. Only one plasmid appeared unique to the host from which it was isolated.

Original languageEnglish
Pages (from-to)138-146
Number of pages9
JournalPlasmid
Volume9
Issue number2
DOIs
Publication statusPublished - 1983

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