Comparison of plasmids in strains of Zymomonas mobilis

H. W. Stokes; E. L. Dally; M. D. Yablonsky; D. E. Eveleigh

doi:10.1016/0147-619X(83)90016-1

Comparison of plasmids in strains of Zymomonas mobilis

H. W. Stokes, E. L. Dally, M. D. Yablonsky, D. E. Eveleigh^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

26 Citations (Scopus)

Abstract

Four strains of Zymomonas mobilis were examined for their resistance to antimicrobial agents and found to have similar resistance profiles. Plasmid DNA was extracted and purified by CsCl dye-buoyant density centrifugation; molecular weights were determined by agarose gel electrophoresis and electron microscopy. All four strains harbored a large plasmid (46 × 10⁶ Da) and a smaller plasmid (16-21 × 10⁶ Da) whose molecular weight was strain dependent. Two strains, Ag11 and ATCC 10988, had smaller plasmids of unique molecular weight. Homology existed between the plasmids in the four strains as shown by cross-reaction in DNA-DNA blot hybridizations. Only one plasmid appeared unique to the host from which it was isolated.

Original language	English
Pages (from-to)	138-146
Number of pages	9
Journal	Plasmid
Volume	9
Issue number	2
DOIs	https://doi.org/10.1016/0147-619X(83)90016-1
Publication status	Published - 1983

Access to Document

10.1016/0147-619X(83)90016-1

Cite this

@article{bba1b650bc0e4ad69ddab1c50b7aa98f,

title = "Comparison of plasmids in strains of Zymomonas mobilis",

abstract = "Four strains of Zymomonas mobilis were examined for their resistance to antimicrobial agents and found to have similar resistance profiles. Plasmid DNA was extracted and purified by CsCl dye-buoyant density centrifugation; molecular weights were determined by agarose gel electrophoresis and electron microscopy. All four strains harbored a large plasmid (46 × 106 Da) and a smaller plasmid (16-21 × 106 Da) whose molecular weight was strain dependent. Two strains, Ag11 and ATCC 10988, had smaller plasmids of unique molecular weight. Homology existed between the plasmids in the four strains as shown by cross-reaction in DNA-DNA blot hybridizations. Only one plasmid appeared unique to the host from which it was isolated.",

author = "Stokes, {H. W.} and Dally, {E. L.} and Yablonsky, {M. D.} and Eveleigh, {D. E.}",

year = "1983",

doi = "10.1016/0147-619X(83)90016-1",

language = "English",

volume = "9",

pages = "138--146",

journal = "Plasmid",

issn = "0147-619X",

publisher = "Academic Press",

number = "2",

}

TY - JOUR

T1 - Comparison of plasmids in strains of Zymomonas mobilis

AU - Stokes, H. W.

AU - Dally, E. L.

AU - Yablonsky, M. D.

AU - Eveleigh, D. E.

PY - 1983

Y1 - 1983

N2 - Four strains of Zymomonas mobilis were examined for their resistance to antimicrobial agents and found to have similar resistance profiles. Plasmid DNA was extracted and purified by CsCl dye-buoyant density centrifugation; molecular weights were determined by agarose gel electrophoresis and electron microscopy. All four strains harbored a large plasmid (46 × 106 Da) and a smaller plasmid (16-21 × 106 Da) whose molecular weight was strain dependent. Two strains, Ag11 and ATCC 10988, had smaller plasmids of unique molecular weight. Homology existed between the plasmids in the four strains as shown by cross-reaction in DNA-DNA blot hybridizations. Only one plasmid appeared unique to the host from which it was isolated.

AB - Four strains of Zymomonas mobilis were examined for their resistance to antimicrobial agents and found to have similar resistance profiles. Plasmid DNA was extracted and purified by CsCl dye-buoyant density centrifugation; molecular weights were determined by agarose gel electrophoresis and electron microscopy. All four strains harbored a large plasmid (46 × 106 Da) and a smaller plasmid (16-21 × 106 Da) whose molecular weight was strain dependent. Two strains, Ag11 and ATCC 10988, had smaller plasmids of unique molecular weight. Homology existed between the plasmids in the four strains as shown by cross-reaction in DNA-DNA blot hybridizations. Only one plasmid appeared unique to the host from which it was isolated.

UR - http://www.scopus.com/inward/record.url?scp=0020529572&partnerID=8YFLogxK

U2 - 10.1016/0147-619X(83)90016-1

DO - 10.1016/0147-619X(83)90016-1

M3 - Article

C2 - 6856691

AN - SCOPUS:0020529572

SN - 0147-619X

VL - 9

SP - 138

EP - 146

JO - Plasmid

JF - Plasmid

IS - 2

ER -

Comparison of plasmids in strains of Zymomonas mobilis

Abstract

Access to Document

Other files and links

Fingerprint

Cite this