Complement factor H: sequence analysis of 221 kb of human genomic DNA containing the entire fH, fHR-1 and fHR-3 genes

Dean A. Male, Rebecca J. Ormsby, Shoba Ranganathan, Eleni Giannakis, David L. Gordon

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Complement factor H (fH) is a member of a family of proteins involved in the regulation of complement activation (RCA). These proteins share a common structural motif, the Short Consensus Repeat (SCR), which is structurally conserved among related genes and between phylogenetically divergent species. fH is composed of 20 such SCRs and a variety of biological functions have been localised to specific SCR domains. The majority of individual SCRs identified are encoded by single exons, and processes such as gene conversion, duplication and exon shuffling have been implicated in the evolution and genomic radiation of SCR-encoding genes. We have analysed two GenBank sequence entries relating to two overlapping PAC clones sequenced at the Sanger Centre which contain the entire human fH gene and two adjacent fH-related (fHR) genes, fHR-1 and fHR-3. Here, we report the detailed analysis of the assembled 221 kb of contiguous, ungapped genomic sequence from human chromosome 1q32, in part employing the RUMMAGE-DP automated annotation tool. Genomic duplications involving fH and fHR exons were identified and Alu/L1 repeat dating established that the duplications occurred after the separation of rodent and primate lineages. The analysis indicates that retrotransposition as well as single and multiple exon duplication events are likely to have been involved in SCR radiation and RCA gene evolution, facilitated by conservation of splice-phasing and the single-exon, single-SCR nature of the encode domains. (C) 2000 Elsevier Science Ltd. All rights reserved.

LanguageEnglish
Pages41-52
Number of pages12
JournalMolecular Immunology
Volume37
Issue number1-2
DOIs
Publication statusPublished - 1 Jan 2000
Externally publishedYes

Fingerprint

Complement Factor H
Sequence Analysis
Exons
Consensus
DNA
Complement Activation
Genes
Radiation
Gene Conversion
Gene Duplication
Nucleic Acid Databases
Human Chromosomes
Primates
Rodentia
Proteins
Clone Cells

Keywords

  • Complement
  • Factor H
  • Factor H-related
  • Gene evolution
  • Gene structure
  • SCR

Cite this

Male, Dean A. ; Ormsby, Rebecca J. ; Ranganathan, Shoba ; Giannakis, Eleni ; Gordon, David L. / Complement factor H : sequence analysis of 221 kb of human genomic DNA containing the entire fH, fHR-1 and fHR-3 genes. In: Molecular Immunology. 2000 ; Vol. 37, No. 1-2. pp. 41-52.
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Complement factor H : sequence analysis of 221 kb of human genomic DNA containing the entire fH, fHR-1 and fHR-3 genes. / Male, Dean A.; Ormsby, Rebecca J.; Ranganathan, Shoba; Giannakis, Eleni; Gordon, David L.

In: Molecular Immunology, Vol. 37, No. 1-2, 01.01.2000, p. 41-52.

Research output: Contribution to journalArticleResearchpeer-review

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