Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen

C. K. Stover; X. Q. Pham; A. L. Erwin; S. D. Mizoguchi; P. Warrener; M. J. Hickey; F. S L Brinkman; W. O. Hufnagle; D. J. Kowallk; M. Lagrou; R. L. Garber; L. Goltry; E. Tolentino; S. Westbrock-Wadman; Y. Yuan; L. L. Brody; S. N. Coulter; K. R. Folger; A. Kas; K. Larbig; R. Lim; K. Smith; D. Spencer; G. K S Wong; Z. Wu; I. T. Paulsen; J. Relzer; M. H. Saler; R. E W Hancock; S. Lory; M. V. Olson

doi:10.1038/35023079

Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen

C. K. Stover, X. Q. Pham, A. L. Erwin, S. D. Mizoguchi, P. Warrener, M. J. Hickey, F. S L Brinkman, W. O. Hufnagle, D. J. Kowallk, M. Lagrou, R. L. Garber, L. Goltry, E. Tolentino, S. Westbrock-Wadman, Y. Yuan, L. L. Brody, S. N. Coulter, K. R. Folger, A. Kas, K. LarbigR. Lim, K. Smith, D. Spencer, G. K S Wong, Z. Wu, I. T. Paulsen, J. Relzer, M. H. Saler, R. E W Hancock, S. Lory, M. V. Olson^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

3651 Citations (Scopus)

Abstract

Pseudomonas aeruginosa is a ubiquitous environmental bacterium that is one of the top three causes of opportunistic human infections. A major factor in its prominence as a pathogen is its intrinsic resistance to antibiotics and disinfectants. Here we report the complete sequence of P. aeruginosa strain PAO1. At 6.3 million base pairs, this is the largest bacterial genome sequenced, and the sequence provides insights into the basis of the versatility and intrinsic drug resistance of P. aeruginosa. Consistent with its larger genome size and environmental adaptability, P. aeruginosa contains the highest proportion of regulatory genes observed for a bacterial genome and a large number of genes involved in the catabolism, transport and efflux of organic compounds as well as four potential chemotaxis systems. We propose that the size and complexity of the P. aeruginosa genome reflect an evolutionary adaptation permitting it to thrive in diverse environments and resist the effects of a variety of antimicrobial substances.

Original language	English
Pages (from-to)	959-964
Number of pages	6
Journal	Nature
Volume	406
Issue number	6799
DOIs	https://doi.org/10.1038/35023079
Publication status	Published - 31 Aug 2000
Externally published	Yes

Access to Document

10.1038/35023079

Cite this

Stover, C. K., Pham, X. Q., Erwin, A. L., Mizoguchi, S. D., Warrener, P., Hickey, M. J., Brinkman, F. S. L., Hufnagle, W. O., Kowallk, D. J., Lagrou, M., Garber, R. L., Goltry, L., Tolentino, E., Westbrock-Wadman, S., Yuan, Y., Brody, L. L., Coulter, S. N., Folger, K. R., Kas, A., ... Olson, M. V. (2000). Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen. Nature, 406(6799), 959-964. https://doi.org/10.1038/35023079

@article{5c8f885d41e143e9b85a9da78583da2b,

title = "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen",

abstract = "Pseudomonas aeruginosa is a ubiquitous environmental bacterium that is one of the top three causes of opportunistic human infections. A major factor in its prominence as a pathogen is its intrinsic resistance to antibiotics and disinfectants. Here we report the complete sequence of P. aeruginosa strain PAO1. At 6.3 million base pairs, this is the largest bacterial genome sequenced, and the sequence provides insights into the basis of the versatility and intrinsic drug resistance of P. aeruginosa. Consistent with its larger genome size and environmental adaptability, P. aeruginosa contains the highest proportion of regulatory genes observed for a bacterial genome and a large number of genes involved in the catabolism, transport and efflux of organic compounds as well as four potential chemotaxis systems. We propose that the size and complexity of the P. aeruginosa genome reflect an evolutionary adaptation permitting it to thrive in diverse environments and resist the effects of a variety of antimicrobial substances.",

author = "Stover, {C. K.} and Pham, {X. Q.} and Erwin, {A. L.} and Mizoguchi, {S. D.} and P. Warrener and Hickey, {M. J.} and Brinkman, {F. S L} and Hufnagle, {W. O.} and Kowallk, {D. J.} and M. Lagrou and Garber, {R. L.} and L. Goltry and E. Tolentino and S. Westbrock-Wadman and Y. Yuan and Brody, {L. L.} and Coulter, {S. N.} and Folger, {K. R.} and A. Kas and K. Larbig and R. Lim and K. Smith and D. Spencer and Wong, {G. K S} and Z. Wu and Paulsen, {I. T.} and J. Relzer and Saler, {M. H.} and Hancock, {R. E W} and S. Lory and Olson, {M. V.}",

year = "2000",

month = aug,

day = "31",

doi = "10.1038/35023079",

language = "English",

volume = "406",

pages = "959--964",

journal = "Nature",

issn = "0028-0836",

publisher = "Nature Publishing Groups",

number = "6799",

}

Stover, CK, Pham, XQ, Erwin, AL, Mizoguchi, SD, Warrener, P, Hickey, MJ, Brinkman, FSL, Hufnagle, WO, Kowallk, DJ, Lagrou, M, Garber, RL, Goltry, L, Tolentino, E, Westbrock-Wadman, S, Yuan, Y, Brody, LL, Coulter, SN, Folger, KR, Kas, A, Larbig, K, Lim, R, Smith, K, Spencer, D, Wong, GKS, Wu, Z, Paulsen, IT, Relzer, J, Saler, MH, Hancock, REW, Lory, S & Olson, MV 2000, 'Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen', Nature, vol. 406, no. 6799, pp. 959-964. https://doi.org/10.1038/35023079

TY - JOUR

T1 - Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen

AU - Stover, C. K.

AU - Pham, X. Q.

AU - Erwin, A. L.

AU - Mizoguchi, S. D.

AU - Warrener, P.

AU - Hickey, M. J.

AU - Brinkman, F. S L

AU - Hufnagle, W. O.

AU - Kowallk, D. J.

AU - Lagrou, M.

AU - Garber, R. L.

AU - Goltry, L.

AU - Tolentino, E.

AU - Westbrock-Wadman, S.

AU - Yuan, Y.

AU - Brody, L. L.

AU - Coulter, S. N.

AU - Folger, K. R.

AU - Kas, A.

AU - Larbig, K.

AU - Lim, R.

AU - Smith, K.

AU - Spencer, D.

AU - Wong, G. K S

AU - Wu, Z.

AU - Paulsen, I. T.

AU - Relzer, J.

AU - Saler, M. H.

AU - Hancock, R. E W

AU - Lory, S.

AU - Olson, M. V.

PY - 2000/8/31

Y1 - 2000/8/31

N2 - Pseudomonas aeruginosa is a ubiquitous environmental bacterium that is one of the top three causes of opportunistic human infections. A major factor in its prominence as a pathogen is its intrinsic resistance to antibiotics and disinfectants. Here we report the complete sequence of P. aeruginosa strain PAO1. At 6.3 million base pairs, this is the largest bacterial genome sequenced, and the sequence provides insights into the basis of the versatility and intrinsic drug resistance of P. aeruginosa. Consistent with its larger genome size and environmental adaptability, P. aeruginosa contains the highest proportion of regulatory genes observed for a bacterial genome and a large number of genes involved in the catabolism, transport and efflux of organic compounds as well as four potential chemotaxis systems. We propose that the size and complexity of the P. aeruginosa genome reflect an evolutionary adaptation permitting it to thrive in diverse environments and resist the effects of a variety of antimicrobial substances.

AB - Pseudomonas aeruginosa is a ubiquitous environmental bacterium that is one of the top three causes of opportunistic human infections. A major factor in its prominence as a pathogen is its intrinsic resistance to antibiotics and disinfectants. Here we report the complete sequence of P. aeruginosa strain PAO1. At 6.3 million base pairs, this is the largest bacterial genome sequenced, and the sequence provides insights into the basis of the versatility and intrinsic drug resistance of P. aeruginosa. Consistent with its larger genome size and environmental adaptability, P. aeruginosa contains the highest proportion of regulatory genes observed for a bacterial genome and a large number of genes involved in the catabolism, transport and efflux of organic compounds as well as four potential chemotaxis systems. We propose that the size and complexity of the P. aeruginosa genome reflect an evolutionary adaptation permitting it to thrive in diverse environments and resist the effects of a variety of antimicrobial substances.

UR - http://www.scopus.com/inward/record.url?scp=0034739007&partnerID=8YFLogxK

U2 - 10.1038/35023079

DO - 10.1038/35023079

M3 - Article

C2 - 10984043

AN - SCOPUS:0034739007

SN - 0028-0836

VL - 406

SP - 959

EP - 964

JO - Nature

JF - Nature

IS - 6799

ER -

Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen

Abstract

Access to Document

Other files and links

Fingerprint

Cite this