TY - JOUR
T1 - Complete genome sequence of the multiresistant taxonomic outlier Pseudomonas aeruginosa PA7
AU - Roy, Paul H.
AU - Tetu, Sasha G.
AU - Larouche, André
AU - Elbourne, Liam
AU - Tremblay, Simon
AU - Ren, Qinghu
AU - Dodson, Robert
AU - Harkins, Derek
AU - Shay, Ryan
AU - Watkins, Kisha
AU - Mahamoud, Yasmin
AU - Paulsen, Ian T.
PY - 2010/1/22
Y1 - 2010/1/22
N2 - Pseudomonas aeruginosa PA7 is a non-respiratory human isolate from Argentina that is multiresistant to antibiotics. We first sequenced gyrA, gyrB, parC, parE, ampC, ampR, and several housekeeping genes and found that PA7 is a taxonomic outlier. We report here the complete sequence of the 6,588,339 bp genome, which has only about 95% overall identity to other strains. PA7 has multiple novel genomic islands and a total of 51 occupied regions of genomic plasticity. These islands include antibiotic resistance genes, parts of transposons, prophages, and a pKLC102-related island. Several PA7 genes not present in PAO1 or PA14 are putative orthologues of other Pseudomonas spp. and Ralstonia spp. genes. PA7 appears to be closely related to the known taxonomic outlier DSM1128 (ATCC9027). PA7 lacks several virulence factors, notably the entire TTSS region corresponding to PA1690-PA1725 of PAO1. It has neither exoS nor exoU and lacks toxA, exoT, and exoY. PA7 is serotype O12 and pyoverdin type II. Preliminary proteomic studies indicate numerous differences with PAO1, some of which are probably a consequence of a frameshift mutation in the mvfR quorum sensing regulatory gene.
AB - Pseudomonas aeruginosa PA7 is a non-respiratory human isolate from Argentina that is multiresistant to antibiotics. We first sequenced gyrA, gyrB, parC, parE, ampC, ampR, and several housekeeping genes and found that PA7 is a taxonomic outlier. We report here the complete sequence of the 6,588,339 bp genome, which has only about 95% overall identity to other strains. PA7 has multiple novel genomic islands and a total of 51 occupied regions of genomic plasticity. These islands include antibiotic resistance genes, parts of transposons, prophages, and a pKLC102-related island. Several PA7 genes not present in PAO1 or PA14 are putative orthologues of other Pseudomonas spp. and Ralstonia spp. genes. PA7 appears to be closely related to the known taxonomic outlier DSM1128 (ATCC9027). PA7 lacks several virulence factors, notably the entire TTSS region corresponding to PA1690-PA1725 of PAO1. It has neither exoS nor exoU and lacks toxA, exoT, and exoY. PA7 is serotype O12 and pyoverdin type II. Preliminary proteomic studies indicate numerous differences with PAO1, some of which are probably a consequence of a frameshift mutation in the mvfR quorum sensing regulatory gene.
UR - http://www.scopus.com/inward/record.url?scp=77749320916&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0008842
DO - 10.1371/journal.pone.0008842
M3 - Article
C2 - 20107499
AN - SCOPUS:77749320916
SN - 1932-6203
VL - 5
SP - 1
EP - 10
JO - PLoS ONE
JF - PLoS ONE
IS - 1
M1 - e8842
ER -