Complete genome sequence of the Q-fever pathogen Coxiella burnetii

Rekha Seshadri; Ian T. Paulsen; Jonathan A. Eisen; Timothy D. Read; Karen E. Nelson; William C. Nelson; Naomi L. Ward; Hervé Tettelin; Tanja M. Davidsen; Maureen J. Beanan; Robert T. Deboy; Sean C. Daugherty; Lauren M. Brinkac; Ramana Madupu; Robert J. Dodson; Hoda M. Khouri; Kathy H. Lee; Heather A. Carty; David Scanlan; Robert A. Heinzen; Herbert A. Thompson; James E. Samuel; Claire M. Fraser; John F. Heidelberg

doi:10.1073/pnas.0931379100

Complete genome sequence of the Q-fever pathogen Coxiella burnetii

Rekha Seshadri, Ian T. Paulsen, Jonathan A. Eisen, Timothy D. Read, Karen E. Nelson, William C. Nelson, Naomi L. Ward, Hervé Tettelin, Tanja M. Davidsen, Maureen J. Beanan, Robert T. Deboy, Sean C. Daugherty, Lauren M. Brinkac, Ramana Madupu, Robert J. Dodson, Hoda M. Khouri, Kathy H. Lee, Heather A. Carty, David Scanlan, Robert A. HeinzenHerbert A. Thompson, James E. Samuel^*, Claire M. Fraser, John F. Heidelberg

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

464 Citations (Scopus)

Abstract

The 1,995,275-bp genome of Coxiella burnetii, Nine Mile phase I RSA493, a highly virulent zoonotic pathogen and category B bioterrorism agent, was sequenced by the random shotgun method. This bacterium is an obligate intracellular acidophile that is highly adapted for life within the eukaryotic phagolysosome. Genome analysis revealed many genes with potential roles in adhesion, invasion, intracellular trafficking, host-cell modulation, and detoxification. A previously uncharacterized 13-member family of ankyrin repeat-containing proteins is implicated in the pathogenesis of this organism. Although the lifestyle and parasitic strategies of C. burnetii resemble that of Rickettsiae and Chlamydiae, their genome architectures differ considerably in terms of presence of mobile elements, extent of genome reduction, metabolic capabilities, and transporter profiles. The presence of 83 pseudogenes displays an ongoing process of gene degradation. Unlike other obligate intracellular bacteria, 32 insertion sequences are found dispersed in the chromosome, indicating some plasticity in the C. burnetii genome. These analyses suggest that the obligate intracellular lifestyle of C. burnetii may be a relatively recent innovation.

Original language	English
Pages (from-to)	5455-5460
Number of pages	6
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	100
Issue number	9
DOIs	https://doi.org/10.1073/pnas.0931379100
Publication status	Published - 29 Apr 2003
Externally published	Yes

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Seshadri, R., Paulsen, I. T., Eisen, J. A., Read, T. D., Nelson, K. E., Nelson, W. C., Ward, N. L., Tettelin, H., Davidsen, T. M., Beanan, M. J., Deboy, R. T., Daugherty, S. C., Brinkac, L. M., Madupu, R., Dodson, R. J., Khouri, H. M., Lee, K. H., Carty, H. A., Scanlan, D., ... Heidelberg, J. F. (2003). Complete genome sequence of the Q-fever pathogen Coxiella burnetii. Proceedings of the National Academy of Sciences of the United States of America, 100(9), 5455-5460. https://doi.org/10.1073/pnas.0931379100

@article{29aa6208142b4fd09940f7e1d93e3fc6,

title = "Complete genome sequence of the Q-fever pathogen Coxiella burnetii",

abstract = "The 1,995,275-bp genome of Coxiella burnetii, Nine Mile phase I RSA493, a highly virulent zoonotic pathogen and category B bioterrorism agent, was sequenced by the random shotgun method. This bacterium is an obligate intracellular acidophile that is highly adapted for life within the eukaryotic phagolysosome. Genome analysis revealed many genes with potential roles in adhesion, invasion, intracellular trafficking, host-cell modulation, and detoxification. A previously uncharacterized 13-member family of ankyrin repeat-containing proteins is implicated in the pathogenesis of this organism. Although the lifestyle and parasitic strategies of C. burnetii resemble that of Rickettsiae and Chlamydiae, their genome architectures differ considerably in terms of presence of mobile elements, extent of genome reduction, metabolic capabilities, and transporter profiles. The presence of 83 pseudogenes displays an ongoing process of gene degradation. Unlike other obligate intracellular bacteria, 32 insertion sequences are found dispersed in the chromosome, indicating some plasticity in the C. burnetii genome. These analyses suggest that the obligate intracellular lifestyle of C. burnetii may be a relatively recent innovation.",

author = "Rekha Seshadri and Paulsen, {Ian T.} and Eisen, {Jonathan A.} and Read, {Timothy D.} and Nelson, {Karen E.} and Nelson, {William C.} and Ward, {Naomi L.} and Herv{\'e} Tettelin and Davidsen, {Tanja M.} and Beanan, {Maureen J.} and Deboy, {Robert T.} and Daugherty, {Sean C.} and Brinkac, {Lauren M.} and Ramana Madupu and Dodson, {Robert J.} and Khouri, {Hoda M.} and Lee, {Kathy H.} and Carty, {Heather A.} and David Scanlan and Heinzen, {Robert A.} and Thompson, {Herbert A.} and Samuel, {James E.} and Fraser, {Claire M.} and Heidelberg, {John F.}",

year = "2003",

month = apr,

day = "29",

doi = "10.1073/pnas.0931379100",

language = "English",

volume = "100",

pages = "5455--5460",

journal = "Proceedings of the National Academy of Sciences of the United States of America",

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Seshadri, R, Paulsen, IT, Eisen, JA, Read, TD, Nelson, KE, Nelson, WC, Ward, NL, Tettelin, H, Davidsen, TM, Beanan, MJ, Deboy, RT, Daugherty, SC, Brinkac, LM, Madupu, R, Dodson, RJ, Khouri, HM, Lee, KH, Carty, HA, Scanlan, D, Heinzen, RA, Thompson, HA, Samuel, JE, Fraser, CM & Heidelberg, JF 2003, 'Complete genome sequence of the Q-fever pathogen Coxiella burnetii', Proceedings of the National Academy of Sciences of the United States of America, vol. 100, no. 9, pp. 5455-5460. https://doi.org/10.1073/pnas.0931379100

TY - JOUR

T1 - Complete genome sequence of the Q-fever pathogen Coxiella burnetii

AU - Seshadri, Rekha

AU - Paulsen, Ian T.

AU - Eisen, Jonathan A.

AU - Read, Timothy D.

AU - Nelson, Karen E.

AU - Nelson, William C.

AU - Ward, Naomi L.

AU - Tettelin, Hervé

AU - Davidsen, Tanja M.

AU - Beanan, Maureen J.

AU - Deboy, Robert T.

AU - Daugherty, Sean C.

AU - Brinkac, Lauren M.

AU - Madupu, Ramana

AU - Dodson, Robert J.

AU - Khouri, Hoda M.

AU - Lee, Kathy H.

AU - Carty, Heather A.

AU - Scanlan, David

AU - Heinzen, Robert A.

AU - Thompson, Herbert A.

AU - Samuel, James E.

AU - Fraser, Claire M.

AU - Heidelberg, John F.

PY - 2003/4/29

Y1 - 2003/4/29

N2 - The 1,995,275-bp genome of Coxiella burnetii, Nine Mile phase I RSA493, a highly virulent zoonotic pathogen and category B bioterrorism agent, was sequenced by the random shotgun method. This bacterium is an obligate intracellular acidophile that is highly adapted for life within the eukaryotic phagolysosome. Genome analysis revealed many genes with potential roles in adhesion, invasion, intracellular trafficking, host-cell modulation, and detoxification. A previously uncharacterized 13-member family of ankyrin repeat-containing proteins is implicated in the pathogenesis of this organism. Although the lifestyle and parasitic strategies of C. burnetii resemble that of Rickettsiae and Chlamydiae, their genome architectures differ considerably in terms of presence of mobile elements, extent of genome reduction, metabolic capabilities, and transporter profiles. The presence of 83 pseudogenes displays an ongoing process of gene degradation. Unlike other obligate intracellular bacteria, 32 insertion sequences are found dispersed in the chromosome, indicating some plasticity in the C. burnetii genome. These analyses suggest that the obligate intracellular lifestyle of C. burnetii may be a relatively recent innovation.

AB - The 1,995,275-bp genome of Coxiella burnetii, Nine Mile phase I RSA493, a highly virulent zoonotic pathogen and category B bioterrorism agent, was sequenced by the random shotgun method. This bacterium is an obligate intracellular acidophile that is highly adapted for life within the eukaryotic phagolysosome. Genome analysis revealed many genes with potential roles in adhesion, invasion, intracellular trafficking, host-cell modulation, and detoxification. A previously uncharacterized 13-member family of ankyrin repeat-containing proteins is implicated in the pathogenesis of this organism. Although the lifestyle and parasitic strategies of C. burnetii resemble that of Rickettsiae and Chlamydiae, their genome architectures differ considerably in terms of presence of mobile elements, extent of genome reduction, metabolic capabilities, and transporter profiles. The presence of 83 pseudogenes displays an ongoing process of gene degradation. Unlike other obligate intracellular bacteria, 32 insertion sequences are found dispersed in the chromosome, indicating some plasticity in the C. burnetii genome. These analyses suggest that the obligate intracellular lifestyle of C. burnetii may be a relatively recent innovation.

UR - http://www.scopus.com/inward/record.url?scp=0038222539&partnerID=8YFLogxK

U2 - 10.1073/pnas.0931379100

DO - 10.1073/pnas.0931379100

M3 - Article

C2 - 12704232

AN - SCOPUS:0038222539

SN - 0027-8424

VL - 100

SP - 5455

EP - 5460

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 9

ER -

Complete genome sequence of the Q-fever pathogen Coxiella burnetii

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