Complete sequence of pJIE143, a pir-type plasmid carrying ISEcp1-bla CTX-M-15 from an Escherichia coli ST131 isolate

Sally R. Partridge; Justin A. Ellem; Sasha G. Tetu; Zhiyong Zong; Ian T. Paulsen; Jonathan R. Iredell

doi:10.1128/AAC.00639-11

Complete sequence of pJIE143, a pir-type plasmid carrying ISEcp1-bla CTX-M-15 from an Escherichia coli ST131 isolate

Sally R. Partridge^*, Justin A. Ellem, Sasha G. Tetu, Zhiyong Zong, Ian T. Paulsen, Jonathan R. Iredell

^*Corresponding author for this work

Macquarie University BioFocus Research Centre

Research output: Contribution to journal › Article › peer-review

36 Citations (Scopus)

Abstract

pJIE143 (34 kb), from an Escherichia coli ST131 isolate, carries bla _CTX-M-15 but could not be typed using the standard PCR-based replicon-typing primer set. Complete sequencing revealed a backbone with similarity to IncX plasmids, including a pir-like gene encoding a π-like replication protein and iterons related to those of other IncX plasmids. The 2.971-kb ISEcp1-bla _CTX-M-15-orf477Δ transposition unit often found within Tn2 is inserted just beyond the end of pir, flanked by 5-bp direct repeats.

Original language	English
Pages (from-to)	5933-5935
Number of pages	3
Journal	Antimicrobial Agents and Chemotherapy
Volume	55
Issue number	12
DOIs	https://doi.org/10.1128/AAC.00639-11
Publication status	Published - Dec 2011

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title = "Complete sequence of pJIE143, a pir-type plasmid carrying ISEcp1-bla CTX-M-15 from an Escherichia coli ST131 isolate",

abstract = "pJIE143 (34 kb), from an Escherichia coli ST131 isolate, carries bla CTX-M-15 but could not be typed using the standard PCR-based replicon-typing primer set. Complete sequencing revealed a backbone with similarity to IncX plasmids, including a pir-like gene encoding a π-like replication protein and iterons related to those of other IncX plasmids. The 2.971-kb ISEcp1-bla CTX-M-15-orf477Δ transposition unit often found within Tn2 is inserted just beyond the end of pir, flanked by 5-bp direct repeats.",

author = "Partridge, {Sally R.} and Ellem, {Justin A.} and Tetu, {Sasha G.} and Zhiyong Zong and Paulsen, {Ian T.} and Iredell, {Jonathan R.}",

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AU - Partridge, Sally R.

AU - Ellem, Justin A.

AU - Tetu, Sasha G.

AU - Zong, Zhiyong

AU - Paulsen, Ian T.

AU - Iredell, Jonathan R.

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AB - pJIE143 (34 kb), from an Escherichia coli ST131 isolate, carries bla CTX-M-15 but could not be typed using the standard PCR-based replicon-typing primer set. Complete sequencing revealed a backbone with similarity to IncX plasmids, including a pir-like gene encoding a π-like replication protein and iterons related to those of other IncX plasmids. The 2.971-kb ISEcp1-bla CTX-M-15-orf477Δ transposition unit often found within Tn2 is inserted just beyond the end of pir, flanked by 5-bp direct repeats.

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Complete sequence of pJIE143, a pir-type plasmid carrying ISEcp1-bla CTX-M-15 from an Escherichia coli ST131 isolate

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