Composition and structure of the 80 S ribosome from the green alga Chlamydomonas reinhardtii

80 S ribosomes are conserved in plants and animals

Andrea L. Manuell, Kenichi Yamaguchi, Paul A. Haynes, Ronald A. Milligan, Stephen P. Mayfield*

*Corresponding author for this work

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

We have conducted a proteomic analysis of the 80 S cytosolic ribosome from the eukaryotic green alga Chlamydomonas reinhardtii, and accompany this with a cryo-electron microscopy structure of the ribosome. Proteins homologous to all but one rat 40 S subunit protein, including a homolog of RACK1, and all but three rat 60 S subunit proteins were identified as components of the C. reinhardtii ribosome. Expressed Sequence Tag (EST) evidence and annotation of the completed C. reinhardtii genome identified genes for each of the four proteins not identified by proteomic analysis, showing that algae potentially have a complete set of orthologs to mammalian 80 S ribosomal proteins. Presented at 25 Å, the algal 80 S ribosome is very similar in structure to the yeast 80 S ribosome, with only minor distinguishable differences. These data show that, although separated by billions of years of evolution, cytosolic ribosomes from photosynthetic organisms are highly conserved with their yeast and animal counterparts.

Original languageEnglish
Pages (from-to)266-279
Number of pages14
JournalJournal of molecular biology
Volume351
Issue number2
DOIs
Publication statusPublished - 12 Aug 2005
Externally publishedYes

Keywords

  • Green algae
  • Proteomic analysis
  • Ribosome
  • Three-dimensional reconstruction
  • Translation

Fingerprint Dive into the research topics of 'Composition and structure of the 80 S ribosome from the green alga Chlamydomonas reinhardtii: 80 S ribosomes are conserved in plants and animals'. Together they form a unique fingerprint.

  • Cite this