Comprehensive chemotaxonomic and genomic profiling of a biosynthetically talented Australian fungus, Aspergillus burnettii sp. nov.

Cameron L. M. Gilchrist; Heather J. Lacey; Daniel Vuong; John I. Pitt; Lene Lange; Ernest Lacey; Bo Pilgaard; Yit-Heng Chooi; Andrew M. Piggott

doi:10.1016/j.fgb.2020.103435

Comprehensive chemotaxonomic and genomic profiling of a biosynthetically talented Australian fungus, Aspergillus burnettii sp. nov.

Cameron L. M. Gilchrist, Heather J. Lacey, Daniel Vuong, John I. Pitt, Lene Lange, Ernest Lacey, Bo Pilgaard, Yit-Heng Chooi^*, Andrew M. Piggott

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

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Abstract

Aspergillus burnettii is a new species belonging to the A. alliaceus clade in Aspergillus subgenus Circumdati section Flavi isolated from peanut-growing properties in southern Queensland, Australia. A. burnettii is a fast-growing, floccose fungus with distinctive brown conidia and is a talented producer of biomass-degrading enzymes and secondary metabolites. Chemical profiling of A. burnettii revealed the metabolites ochratoxin A, kotanins, isokotanins, asperlicin E, anominine and paspalinine, which are common to subgenus Circumdati, together with burnettiene A, burnettramic acids, burnettides, and high levels of 14α-hydroxypaspalinine and hirsutide. The genome of A. burnettii was sequenced and an annotated draft genome is presented. A. burnettii is rich in secondary metabolite biosynthetic gene clusters, containing 51 polyketide synthases, 28 non-ribosomal peptide synthetases and 19 genes related to terpene biosynthesis. Functional annotation of digestive enzymes of A. burnettii and A. alliaceus revealed overlapping carbon utilisation profiles, consistent with a close phylogenetic relationship.

Original language	English
Article number	103435
Pages (from-to)	1-15
Number of pages	15
Journal	Fungal Genetics and Biology
Volume	143
DOIs	https://doi.org/10.1016/j.fgb.2020.103435
Publication status	Published - Oct 2020

Keywords

Aspergillus
Biosynthetic gene cluster analysis
Carbohydrate active enzymes
Chemotaxonomy
Molecular phylogeny
Secondary metabolites

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10.1016/j.fgb.2020.103435

Accepted author manuscriptAccepted author manuscript, 9.61 MBLicence: CC BY-NC-ND

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title = "Comprehensive chemotaxonomic and genomic profiling of a biosynthetically talented Australian fungus, Aspergillus burnettii sp. nov.",

abstract = "Aspergillus burnettii is a new species belonging to the A. alliaceus clade in Aspergillus subgenus Circumdati section Flavi isolated from peanut-growing properties in southern Queensland, Australia. A. burnettii is a fast-growing, floccose fungus with distinctive brown conidia and is a talented producer of biomass-degrading enzymes and secondary metabolites. Chemical profiling of A. burnettii revealed the metabolites ochratoxin A, kotanins, isokotanins, asperlicin E, anominine and paspalinine, which are common to subgenus Circumdati, together with burnettiene A, burnettramic acids, burnettides, and high levels of 14α-hydroxypaspalinine and hirsutide. The genome of A. burnettii was sequenced and an annotated draft genome is presented. A. burnettii is rich in secondary metabolite biosynthetic gene clusters, containing 51 polyketide synthases, 28 non-ribosomal peptide synthetases and 19 genes related to terpene biosynthesis. Functional annotation of digestive enzymes of A. burnettii and A. alliaceus revealed overlapping carbon utilisation profiles, consistent with a close phylogenetic relationship.",

keywords = "Aspergillus, Biosynthetic gene cluster analysis, Carbohydrate active enzymes, Chemotaxonomy, Molecular phylogeny, Secondary metabolites",

author = "Gilchrist, {Cameron L. M.} and Lacey, {Heather J.} and Daniel Vuong and Pitt, {John I.} and Lene Lange and Ernest Lacey and Bo Pilgaard and Yit-Heng Chooi and Piggott, {Andrew M.}",

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AU - Gilchrist, Cameron L. M.

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AU - Pitt, John I.

AU - Lange, Lene

AU - Lacey, Ernest

AU - Pilgaard, Bo

AU - Chooi, Yit-Heng

AU - Piggott, Andrew M.

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AB - Aspergillus burnettii is a new species belonging to the A. alliaceus clade in Aspergillus subgenus Circumdati section Flavi isolated from peanut-growing properties in southern Queensland, Australia. A. burnettii is a fast-growing, floccose fungus with distinctive brown conidia and is a talented producer of biomass-degrading enzymes and secondary metabolites. Chemical profiling of A. burnettii revealed the metabolites ochratoxin A, kotanins, isokotanins, asperlicin E, anominine and paspalinine, which are common to subgenus Circumdati, together with burnettiene A, burnettramic acids, burnettides, and high levels of 14α-hydroxypaspalinine and hirsutide. The genome of A. burnettii was sequenced and an annotated draft genome is presented. A. burnettii is rich in secondary metabolite biosynthetic gene clusters, containing 51 polyketide synthases, 28 non-ribosomal peptide synthetases and 19 genes related to terpene biosynthesis. Functional annotation of digestive enzymes of A. burnettii and A. alliaceus revealed overlapping carbon utilisation profiles, consistent with a close phylogenetic relationship.

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Comprehensive chemotaxonomic and genomic profiling of a biosynthetically talented Australian fungus, Aspergillus burnettii sp. nov.

Abstract

Keywords

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BioAustralis, towards the future

Overcoming antibiotic resistance: rapid discovery of new antibacterial drug targets using chemicalproteomics

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Comprehensive chemotaxonomic and genomic profiling of a biosynthetically talented Australian fungus, Aspergillus burnettii sp. nov.

Abstract

Keywords

Access to Document

Other files and links

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BioAustralis, towards the future

Overcoming antibiotic resistance: rapid discovery of new antibacterial drug targets using chemicalproteomics

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