Comprehensive gene expression analysis of human embryonic stem cells during differentiation into neural cells

Ali Fathi, Maryam Hatami, Vahid Hajihosseini, Faranak Fattahi, Sahar Kiani, Hossein Baharvand*, Ghasem Hosseini Salekdeh

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

49 Citations (Scopus)
11 Downloads (Pure)

Abstract

Global gene expression analysis of human embryonic stem cells (hESCs) that differentiate into neural cells would help to further define the molecular mechanisms involved in neurogenesis in humans. We performed a comprehensive transcripteome analysis of hESC differentiation at three different stages: early neural differentiation, neural ectoderm, and differentiated neurons. We identified and validated time-dependent gene expression patterns and showed that the gene expression patterns reflect early ESC differentiation. Sets of genes are induced in primary ectodermal lineages and then in differentiated neurons, constituting consecutive waves of known and novel genes. Pathway analysis revealed dynamic expression patterns of members of several signaling pathways, including NOTCH, mTOR and Toll like receptors (TLR), during neural differentiation. An interaction network analysis revealed that the TGFβ family of genes, including LEFTY1, ID1 and ID2, are possible key players in the proliferation and maintenance of neural ectoderm. Collectively, these results enhance our understanding of the molecular dynamics underlying neural commitment and differentiation.

Original languageEnglish
Article numbere22856
Pages (from-to)1-13
Number of pages13
JournalPLoS ONE
Volume6
Issue number7
DOIs
Publication statusPublished - 2 Aug 2011
Externally publishedYes

Bibliographical note

Copyright the Author(s) 2011. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

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