Confocal microscopy of FM 4-64 tagged membranes in the living fungus Trichoderma reesei

Hong Yu*, Debra Birch, Junior Te'o, Ewa Goldys, Helena Nevalainen

*Corresponding author for this work

    Research output: Contribution to journalMeeting abstract

    Abstract

    Visualization of the protein secretory pathway in the high secreting trichoderma reesei (T. reesei) hyphae using confocal microscopy will lead to better understanding of the cellular mechanisms involved in protein secretion and contribute to the identification of bottlenecks in the secretion of foreign proteins from fungi. An introduction into visualization approaches involved the application of the fluorescent dye FM 4-64 for staining of membrane-based structures in T. reesei hyphae. Confocal microscopy studies were carried out with 24-hour old cultures of the T. reesei strain Rut-C30. Staining of the fungal hyphae and characteristics of FM 4-64 including spectral properties, time course of the labeling and double labeling with other specific organelle dyes will be discussed.

    Original languageEnglish
    Pages (from-to)S143-S146
    Number of pages4
    JournalChinese Optics Letters
    Volume3
    Issue numberS1
    Publication statusPublished - Aug 2005
    EventInternational Symposium on Photonics, Biophotonics and Nanophotonics - Nanjing, China
    Duration: 14 May 200518 May 2005

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