Constructing a structural model of troponin using site-directed spin labeling: EPR and PRE-NMR

Research output: Contribution to journalReview articleResearchpeer-review

Abstract

The relative ease of introducing a paramagnetic species onto a protein, and advances in electron paramagnetic resonance (EPR) over the past two decades, have established spin labeling as a vital structural biology technique for revealing the functional workings of the troponin muscle regulatory complex—an ~80 kDa heterotrimeric protein switch for turning on striated muscle contraction. Through the site-directed spin labeling (SDSL) of cysteine residues at key sites in troponin, a molecular-level understanding of the troponin muscle regulatory system across all levels of structural hierarchy has been achieved. Through the application of EPR, mobility and accessibility trends in the EPR signals of the spin labels attached to consecutive residues can reveal the secondary structure of troponin elements and also help map the interaction between subunits. Distance restraints calculated from the interspin interactions between spin label pairs have helped with building a structural model of the troponin complex. Further, when SDSL is paired with NMR, paramagnetic relaxation enhancement (PRE)-NMR has been used to obtain high-resolution structural detail for both intra- and interdomain interactions in troponin and revealed details of protein conformational changes and dynamics accompanying troponin function. In this review, we provide an overview of the SDSL labeling methodology and its application towards building a dynamic structural model of the multi-subunit troponin complex which details the calcium-induced conformational changes intimately linked to muscle regulation. We also describe how the SDSL method, in conjunction with EPR or NMR, can be used to obtain insights into structural perturbations to troponin caused by disease-causing mutations.

LanguageEnglish
Pages621-639
Number of pages19
JournalBiophysical Reviews
Volume11
Issue number4
DOIs
Publication statusPublished - Aug 2019

Fingerprint

Troponin
Structural Models
Electron Spin Resonance Spectroscopy
Spin Labels
Muscles
Proteins
Striated Muscle
Muscle Contraction
Cysteine
Calcium
Mutation

Keywords

  • Cardiac troponin
  • Site-directed spin labeling
  • Electron paramagnetic resonance (EPR)
  • Paramagnetic relaxation enhancementNMR (PRE-NMR)
  • Protein dynamics

Cite this

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title = "Constructing a structural model of troponin using site-directed spin labeling: EPR and PRE-NMR",
abstract = "The relative ease of introducing a paramagnetic species onto a protein, and advances in electron paramagnetic resonance (EPR) over the past two decades, have established spin labeling as a vital structural biology technique for revealing the functional workings of the troponin muscle regulatory complex—an ~80 kDa heterotrimeric protein switch for turning on striated muscle contraction. Through the site-directed spin labeling (SDSL) of cysteine residues at key sites in troponin, a molecular-level understanding of the troponin muscle regulatory system across all levels of structural hierarchy has been achieved. Through the application of EPR, mobility and accessibility trends in the EPR signals of the spin labels attached to consecutive residues can reveal the secondary structure of troponin elements and also help map the interaction between subunits. Distance restraints calculated from the interspin interactions between spin label pairs have helped with building a structural model of the troponin complex. Further, when SDSL is paired with NMR, paramagnetic relaxation enhancement (PRE)-NMR has been used to obtain high-resolution structural detail for both intra- and interdomain interactions in troponin and revealed details of protein conformational changes and dynamics accompanying troponin function. In this review, we provide an overview of the SDSL labeling methodology and its application towards building a dynamic structural model of the multi-subunit troponin complex which details the calcium-induced conformational changes intimately linked to muscle regulation. We also describe how the SDSL method, in conjunction with EPR or NMR, can be used to obtain insights into structural perturbations to troponin caused by disease-causing mutations.",
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Constructing a structural model of troponin using site-directed spin labeling : EPR and PRE-NMR. / Kachooei, Ehsan; Cordina, Nicole M.; Brown, Louise J.

In: Biophysical Reviews, Vol. 11, No. 4, 08.2019, p. 621-639.

Research output: Contribution to journalReview articleResearchpeer-review

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