Controlled expression of the dominant flocculation genes FLO1, FLO5, and FLO11 in Saccharomyces cerevisiae

Patrick Govender, Jody L. Domingo, Michael C. Bester, Isak S. Pretorius, Florian F. Bauer

Research output: Contribution to journalArticleResearchpeer-review

Abstract

In many industrial fermentation processes, the Saccharomyces cerevisiae yeast should ideally meet two partially conflicting demands. During fermentation, a high suspended yeast count is required to maintain a satisfactory rate of fermentation, while at completion, efficient settling is desired to enhance product clarification and recovery. In most fermentation industries, currently used starter cultures do not satisfy this ideal, probably because nonflocculent yeast strains were selected to avoid fermentation problems. In this paper, we assess molecular strategies to optimize the flocculation behavior of S. cerevisiae. For this purpose, the chromosomal copies of three dominant flocculation genes, FLO1, FLO5, and FLO11, of the haploid nonflocculent, noninvasive, and non-flor-forming S. cerevisiae FY23 strain were placed under the transcriptional control of the promoters of the ADH2 and HSP30 genes. All six promoter-gene combinations resulted in specific flocculation behaviors in terms of timing and intensity. The strategy resulted in stable expression patterns providing a platform for the direct comparison and assessment of the specific impact of the expression of individual dominant FLO genes with regard to cell wall characteristics, such as hydrophobicity, biofilm formation, and substrate adhesion properties. The data also clearly demonstrate that the flocculation behavior of yeast strains can be tightly controlled and fine-tuned to satisfy specific industrial requirements.

LanguageEnglish
Pages6041-6052
Number of pages12
JournalApplied and Environmental Microbiology
Volume74
Issue number19
DOIs
Publication statusPublished - Oct 2008
Externally publishedYes

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Flocculation
Dominant Genes
flocculation
Fermentation
fermentation
Saccharomyces cerevisiae
yeast
yeasts
Yeasts
gene
genes
fermentation industry
promoter regions
dominant genes
starter cultures
hydrophobicity
haploidy
biofilm
Haploidy
adhesion

Cite this

Govender, Patrick ; Domingo, Jody L. ; Bester, Michael C. ; Pretorius, Isak S. ; Bauer, Florian F. / Controlled expression of the dominant flocculation genes FLO1, FLO5, and FLO11 in Saccharomyces cerevisiae. In: Applied and Environmental Microbiology. 2008 ; Vol. 74, No. 19. pp. 6041-6052.
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Controlled expression of the dominant flocculation genes FLO1, FLO5, and FLO11 in Saccharomyces cerevisiae. / Govender, Patrick; Domingo, Jody L.; Bester, Michael C.; Pretorius, Isak S.; Bauer, Florian F.

In: Applied and Environmental Microbiology, Vol. 74, No. 19, 10.2008, p. 6041-6052.

Research output: Contribution to journalArticleResearchpeer-review

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