Controlling protein orientation at interfaces using histidine tags

An alternative to Ni/NTA

Daniel L. Johnson, Lisandra L. Martin*

*Corresponding author for this work

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

A strategy for immobilizing histidine-tagged proteins at surfaces has been developed by using a macrocyclic chelator that enhances the stability and specificity of conventional histidine-tag technology and allows application of this technology to long-term studies including direct electrochemistry.

Original languageEnglish
Pages (from-to)2018-2019
Number of pages2
JournalJournal of the American Chemical Society
Volume127
Issue number7
DOIs
Publication statusPublished - 23 Feb 2005
Externally publishedYes

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