Controlling protein orientation at interfaces using histidine tags: An alternative to Ni/NTA

Daniel L. Johnson; Lisandra L. Martin

doi:10.1021/ja045084g

Controlling protein orientation at interfaces using histidine tags: An alternative to Ni/NTA

Daniel L. Johnson, Lisandra L. Martin^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

96 Citations (Scopus)

Abstract

A strategy for immobilizing histidine-tagged proteins at surfaces has been developed by using a macrocyclic chelator that enhances the stability and specificity of conventional histidine-tag technology and allows application of this technology to long-term studies including direct electrochemistry.

Original language	English
Pages (from-to)	2018-2019
Number of pages	2
Journal	Journal of the American Chemical Society
Volume	127
Issue number	7
DOIs	https://doi.org/10.1021/ja045084g
Publication status	Published - 23 Feb 2005
Externally published	Yes

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10.1021/ja045084g

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title = "Controlling protein orientation at interfaces using histidine tags: An alternative to Ni/NTA",

abstract = "A strategy for immobilizing histidine-tagged proteins at surfaces has been developed by using a macrocyclic chelator that enhances the stability and specificity of conventional histidine-tag technology and allows application of this technology to long-term studies including direct electrochemistry.",

author = "Johnson, \{Daniel L.\} and Martin, \{Lisandra L.\}",

year = "2005",

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AU - Martin, Lisandra L.

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N2 - A strategy for immobilizing histidine-tagged proteins at surfaces has been developed by using a macrocyclic chelator that enhances the stability and specificity of conventional histidine-tag technology and allows application of this technology to long-term studies including direct electrochemistry.

AB - A strategy for immobilizing histidine-tagged proteins at surfaces has been developed by using a macrocyclic chelator that enhances the stability and specificity of conventional histidine-tag technology and allows application of this technology to long-term studies including direct electrochemistry.

UR - https://www.scopus.com/pages/publications/13944267055

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DO - 10.1021/ja045084g

M3 - Article

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AN - SCOPUS:13944267055

SN - 0002-7863

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SP - 2018

EP - 2019

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

IS - 7

ER -

Controlling protein orientation at interfaces using histidine tags: An alternative to Ni/NTA

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