Conventional Approaches for Sample Preparation for Liquid Chromatography and Two-Dimensional Gel Electrophoresis

Vesela Encheva*, Robert Parker

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (Scopus)
Original languageEnglish
Title of host publicationMass Spectrometry for Microbial Proteomics
PublisherJohn Wiley & Sons
Pages117-142
Number of pages26
ISBN (Print)9780470681992
DOIs
Publication statusPublished - 15 Jun 2010

Keywords

  • 2D GE-mark on elucidating mechanisms of bacterial pathogenicity, antibiotics resistance, physiology and vaccine development
  • Cell lysis methods and mechanical lysis
  • Chemical and osmotic lysis
  • Fractionation strategies and surface associated proteins
  • French pressure cell, most effective systems for cell disruption of bacterial cells
  • Microscale clean up prior to LC-MS
  • Protein samples-preparation techniques
  • Sample preparation for 2D GE
  • Sample preparation for liquid chromatography and two-dimensional gel electrophoresis-conventional approaches
  • Sonication, applying sound energy-to agitate particles in sample

Cite this