Conventional Approaches for Sample Preparation for Liquid Chromatography and Two-Dimensional Gel Electrophoresis

Vesela Encheva*, Robert Parker

*Corresponding author for this work

    Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

    2 Citations (Scopus)
    Original languageEnglish
    Title of host publicationMass Spectrometry for Microbial Proteomics
    PublisherJohn Wiley & Sons
    Pages117-142
    Number of pages26
    ISBN (Print)9780470681992
    DOIs
    Publication statusPublished - 15 Jun 2010

    Keywords

    • 2D GE-mark on elucidating mechanisms of bacterial pathogenicity, antibiotics resistance, physiology and vaccine development
    • Cell lysis methods and mechanical lysis
    • Chemical and osmotic lysis
    • Fractionation strategies and surface associated proteins
    • French pressure cell, most effective systems for cell disruption of bacterial cells
    • Microscale clean up prior to LC-MS
    • Protein samples-preparation techniques
    • Sample preparation for 2D GE
    • Sample preparation for liquid chromatography and two-dimensional gel electrophoresis-conventional approaches
    • Sonication, applying sound energy-to agitate particles in sample

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