Co‐operative effect between insulin‐like growth factor‐1 and interleukin‐2 on DNA synthesis and interleukin‐2 receptor‐α chain expression in human lymphocytes

ROXANA SCHILLACI, MARIANA G. BROCARDO, ALICIA ROLDAN

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Recently we demonstrated that IGF‐1 has the same effect on DNA synthesis and G1‐phase length during human lymphocyte proliferation as IL‐2. In order to determine the link between IGF‐1 and IL‐2 on lymphocyte proliferation, we tested the ability of these cytokines, alone or in combination, to induce DNA synthesis and increase the number of cells expressing IL‐2‐α chain receptors. Our results showed that the increase in DNA synthesis produced by the addition of IGF‐1 to cells cultured with saturable concentrations of IL‐2, correlated well with an increase in the number of cells expressing IL‐2‐α chain receptor. The effect on both DNA synthesis and IL‐2‐α chain receptor expression was greater at lower concentrations of PHA. This study suggests that the proliferative effect of IGF‐1 might be due to separate stimulation of different cell populations and/or by activation of a tyrosine‐kinase signal transduction pathway complementary to the IL‐2 activation signal.

LanguageEnglish
Pages340-345
Number of pages6
JournalImmunology and Cell Biology
Volume73
Issue number4
DOIs
Publication statusPublished - 1995
Externally publishedYes

Fingerprint

Interleukin-2 Receptor alpha Subunit
Somatomedins
Interleukin-2
Lymphocytes
Insulin-Like Growth Factor I
DNA
Cell Count
G1 Phase
Protein-Tyrosine Kinases
Cultured Cells
Signal Transduction
Cytokines

Keywords

  • chain receptor
  • IGF‐1
  • IL‐2
  • IL‐2‐α
  • Key words: DNA
  • lymphocytes
  • peripheral blood mononuclear cells
  • PHA

Cite this

@article{1a000623adc94dc4bde6ed050caf2b5f,
title = "Co‐operative effect between insulin‐like growth factor‐1 and interleukin‐2 on DNA synthesis and interleukin‐2 receptor‐α chain expression in human lymphocytes",
abstract = "Recently we demonstrated that IGF‐1 has the same effect on DNA synthesis and G1‐phase length during human lymphocyte proliferation as IL‐2. In order to determine the link between IGF‐1 and IL‐2 on lymphocyte proliferation, we tested the ability of these cytokines, alone or in combination, to induce DNA synthesis and increase the number of cells expressing IL‐2‐α chain receptors. Our results showed that the increase in DNA synthesis produced by the addition of IGF‐1 to cells cultured with saturable concentrations of IL‐2, correlated well with an increase in the number of cells expressing IL‐2‐α chain receptor. The effect on both DNA synthesis and IL‐2‐α chain receptor expression was greater at lower concentrations of PHA. This study suggests that the proliferative effect of IGF‐1 might be due to separate stimulation of different cell populations and/or by activation of a tyrosine‐kinase signal transduction pathway complementary to the IL‐2 activation signal.",
keywords = "chain receptor, IGF‐1, IL‐2, IL‐2‐α, Key words: DNA, lymphocytes, peripheral blood mononuclear cells, PHA",
author = "ROXANA SCHILLACI and BROCARDO, {MARIANA G.} and ALICIA ROLDAN",
year = "1995",
doi = "10.1111/j.1440-1711.1995.tb03877.x",
language = "English",
volume = "73",
pages = "340--345",
journal = "Immunology and Cell Biology",
issn = "0818-9641",
publisher = "Blackwell Publishing",
number = "4",

}

Co‐operative effect between insulin‐like growth factor‐1 and interleukin‐2 on DNA synthesis and interleukin‐2 receptor‐α chain expression in human lymphocytes. / SCHILLACI, ROXANA; BROCARDO, MARIANA G.; ROLDAN, ALICIA.

In: Immunology and Cell Biology, Vol. 73, No. 4, 1995, p. 340-345.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Co‐operative effect between insulin‐like growth factor‐1 and interleukin‐2 on DNA synthesis and interleukin‐2 receptor‐α chain expression in human lymphocytes

AU - SCHILLACI, ROXANA

AU - BROCARDO, MARIANA G.

AU - ROLDAN, ALICIA

PY - 1995

Y1 - 1995

N2 - Recently we demonstrated that IGF‐1 has the same effect on DNA synthesis and G1‐phase length during human lymphocyte proliferation as IL‐2. In order to determine the link between IGF‐1 and IL‐2 on lymphocyte proliferation, we tested the ability of these cytokines, alone or in combination, to induce DNA synthesis and increase the number of cells expressing IL‐2‐α chain receptors. Our results showed that the increase in DNA synthesis produced by the addition of IGF‐1 to cells cultured with saturable concentrations of IL‐2, correlated well with an increase in the number of cells expressing IL‐2‐α chain receptor. The effect on both DNA synthesis and IL‐2‐α chain receptor expression was greater at lower concentrations of PHA. This study suggests that the proliferative effect of IGF‐1 might be due to separate stimulation of different cell populations and/or by activation of a tyrosine‐kinase signal transduction pathway complementary to the IL‐2 activation signal.

AB - Recently we demonstrated that IGF‐1 has the same effect on DNA synthesis and G1‐phase length during human lymphocyte proliferation as IL‐2. In order to determine the link between IGF‐1 and IL‐2 on lymphocyte proliferation, we tested the ability of these cytokines, alone or in combination, to induce DNA synthesis and increase the number of cells expressing IL‐2‐α chain receptors. Our results showed that the increase in DNA synthesis produced by the addition of IGF‐1 to cells cultured with saturable concentrations of IL‐2, correlated well with an increase in the number of cells expressing IL‐2‐α chain receptor. The effect on both DNA synthesis and IL‐2‐α chain receptor expression was greater at lower concentrations of PHA. This study suggests that the proliferative effect of IGF‐1 might be due to separate stimulation of different cell populations and/or by activation of a tyrosine‐kinase signal transduction pathway complementary to the IL‐2 activation signal.

KW - chain receptor

KW - IGF‐1

KW - IL‐2

KW - IL‐2‐α

KW - Key words: DNA

KW - lymphocytes

KW - peripheral blood mononuclear cells

KW - PHA

UR - http://www.scopus.com/inward/record.url?scp=0029115075&partnerID=8YFLogxK

U2 - 10.1111/j.1440-1711.1995.tb03877.x

DO - 10.1111/j.1440-1711.1995.tb03877.x

M3 - Article

VL - 73

SP - 340

EP - 345

JO - Immunology and Cell Biology

T2 - Immunology and Cell Biology

JF - Immunology and Cell Biology

SN - 0818-9641

IS - 4

ER -