Coregulation of starch degradation and dimorphism in the yeast Saccharomyces cerevisiae

Melané A. Vivier, Marius G. Lambrechts, Isak S. Pretorius

Research output: Contribution to journalReview articleResearchpeer-review

Abstract

Saccharomyces cerevisiae, the exemplar unicellular eukaryote, can only survive and proliferate in its natural habitats through constant adaptation with the constraints of a dynamic ecosystem. In every cell cycle of S. cerevisiae, there is a short period in the G1 phase of the cell cycle where 'sensing' transpires; if a sufficient amount of fermentable sugars is available, the cells will initiate another round of vegetative cell division. When fermentable sugars become limiting, the yeast can execute the diauxic shift, where it reprograms its metabolism to utilize nonfermentable carbon sources. S. cerevisiae can also initiate the developmental program of pseudohyphal formation and invasive growth response, when essential nutrients become limiting. S. cerevisiae shares this growth form-switching ability with important pathogens such as the human pathogen, Candida albicans, and the corn smut pathogen Ustilago maydis. The pseudohyphal growth response of S. cerevisiae has mainly been implicated as a means for the yeast to search for nutrients. An important observation made was that starch-degrading S. cerevisiae strains have the added ability to form pseudohyphae and grow invasively into a starch-containing medium. More significantly, it was also shown that the STA1-3 gene encoding three glucoamylase isozymes responsible for starch hydrolysis in S. cerevisiae are coregulated with a gene, MUC1, essential for pseudohyphal and invasive growth. At least two putative transcriptional activators, Mss10p and Mss11p, are involved in this regulation. The Muc1p is a putative integral membrane-bound protein similar to mammalian mucin-like proteins that have been implicated in the ability of cancer cells to invade other tissues. This provided us with an excellent example of integrative control between nutrient sensing, signaling, and differential development.

LanguageEnglish
Pages405-435
Number of pages31
JournalCritical Reviews in Biochemistry and Molecular Biology
Volume32
Issue number5
Publication statusPublished - 1997
Externally publishedYes

Fingerprint

Starch
Yeast
Saccharomyces cerevisiae
Yeasts
Degradation
Pathogens
Cells
Nutrients
Growth
Food
Ecosystem
Cell Cycle
Sugars
Ustilago
Glucan 1,4-alpha-Glucosidase
Essential Genes
G1 Phase
Mucins
Eukaryota
Candida albicans

Cite this

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title = "Coregulation of starch degradation and dimorphism in the yeast Saccharomyces cerevisiae",
abstract = "Saccharomyces cerevisiae, the exemplar unicellular eukaryote, can only survive and proliferate in its natural habitats through constant adaptation with the constraints of a dynamic ecosystem. In every cell cycle of S. cerevisiae, there is a short period in the G1 phase of the cell cycle where 'sensing' transpires; if a sufficient amount of fermentable sugars is available, the cells will initiate another round of vegetative cell division. When fermentable sugars become limiting, the yeast can execute the diauxic shift, where it reprograms its metabolism to utilize nonfermentable carbon sources. S. cerevisiae can also initiate the developmental program of pseudohyphal formation and invasive growth response, when essential nutrients become limiting. S. cerevisiae shares this growth form-switching ability with important pathogens such as the human pathogen, Candida albicans, and the corn smut pathogen Ustilago maydis. The pseudohyphal growth response of S. cerevisiae has mainly been implicated as a means for the yeast to search for nutrients. An important observation made was that starch-degrading S. cerevisiae strains have the added ability to form pseudohyphae and grow invasively into a starch-containing medium. More significantly, it was also shown that the STA1-3 gene encoding three glucoamylase isozymes responsible for starch hydrolysis in S. cerevisiae are coregulated with a gene, MUC1, essential for pseudohyphal and invasive growth. At least two putative transcriptional activators, Mss10p and Mss11p, are involved in this regulation. The Muc1p is a putative integral membrane-bound protein similar to mammalian mucin-like proteins that have been implicated in the ability of cancer cells to invade other tissues. This provided us with an excellent example of integrative control between nutrient sensing, signaling, and differential development.",
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Coregulation of starch degradation and dimorphism in the yeast Saccharomyces cerevisiae. / Vivier, Melané A.; Lambrechts, Marius G.; Pretorius, Isak S.

In: Critical Reviews in Biochemistry and Molecular Biology, Vol. 32, No. 5, 1997, p. 405-435.

Research output: Contribution to journalReview articleResearchpeer-review

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