Corticosterone-immune interactions during captive stress in invading Australian cane toads (Rhinella marina)

Sean P. Graham*, Crystal Kelehear, Gregory P. Brown, Richard Shine

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

38 Citations (Scopus)

Abstract

Vertebrates cope with physiological challenges using two major mechanisms: the immune system and the hypothalamic pituitary-adrenal axis (e.g., the glucocorticoid stress response). Because the two systems are tightly integrated, we need simultaneous studies of both systems, in a range of species, to understand how vertebrates respond to novel challenges. To clarify how glucocorticoids modulate the amphibian immune system, we measured three immune parameters and plasma corticosterone (CORT), before and after inflicting a stressor (capture and captive confinement) on introduced cane toads (Rhinella marina) near their invasion front in Australia. Stress increased CORT levels, decreased complement lysis capacity, increased leukocyte oxidative burst, and did not change heterologous erythrocyte agglutination. The strength of the CORT response was positively correlated with leukocyte oxidative burst, and morphological features associated with invasiveness in cane toads (relative leg length) were correlated with stress responsiveness. No immune parameter that we measured was affected by a toad's infection by a parasitic nematode (Rhabdias pseudosphaerocephala), but the CORT response was muted in infected versus uninfected toads. These results illustrate the complex immune-stress interactions in wild populations of a non-traditional model vertebrate species, and describe immune adaptations of an important invasive species.

Original languageEnglish
Pages (from-to)146-153
Number of pages8
JournalHormones and Behavior
Volume62
Issue number2
DOIs
Publication statusPublished - Jul 2012
Externally publishedYes

Keywords

  • amphibian
  • anthropogenic stressor
  • complement
  • HPA axis
  • innate immunity
  • invasive species
  • phagocytosis
  • Rhabdias pseudosphaerocephala

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