Cospray dried antibiotics for dry powder lung delivery

Handoko Adi, Paul M. Young, Hak-Kim Chan, Peter Stewart, Helen Agus, Daniela Traini

Research output: Contribution to journalArticlepeer-review

57 Citations (Scopus)

Abstract

The aim of this study was to assess the potential of delivering a combination antibiotic therapy, containing doxycycline and ciprofloxacin (both hydrochloride) as a dry powder (DPI) formulation for inhalation. Single and combination antibiotics were produced by spray drying. Particle size distributions were characterized by laser diffraction and imaging conducted by scanning electron microscopy. Solid-state characterisation of the antibiotics was carried out using differential scanning calorimetry, dynamic vapour sorption, X-ray powder diffraction, and differential scanning calorimetry. Using the Aerolizer® device, the aerosol performance was measured using multistage liquid impinger and analysed using high performance liquid chromatography (R2 = 1.0, CV = 0.4–1.0%). Furthermore, a disk diffusion test was performed for the assessment of the in vitro antimicrobial activity of the raw and spray dried antibiotics against bacteria. Results showed that cospray drying of the ciprofloxacin and doxycycline produced an antibiotic formulation (in a 1:1 ratio) suitable for inhalation that showed to be physically more stable then the analogous single spray dried antibiotic. The cospray dried powder has improved dispersion over the less stable single spray dried ciprofloxacin. The spray dried antibiotics were observed to have similar antimicrobial activity to the original antibiotics for Staphylococcus aureus, Pseudomonas aeruginosa and Streptococcus pyrogenes, suggesting the spray drying process does not affect the anti-bacterial activity of the drug. Cospray dried antibiotics from a DPI is thus feasible and can potentially be an attractive delivery alternative to the more conventional systemic delivery route.
Original languageEnglish
Pages (from-to)3356-3366
Number of pages11
JournalJournal of Pharmaceutical Sciences
Volume97
Issue number8
DOIs
Publication statusPublished - Aug 2008
Externally publishedYes

Keywords

  • dry powder inhaler
  • DPI
  • antibiotics
  • doxycycline hydrochloride
  • ciprofloxacin hydrochloride
  • cospray drying

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