Covalent fabrication of methyl parathion hydrolase on gold nanoparticles modified carbon substrates for designing a methyl parathion biosensor

Guozhen Liu*, Wenqi Guo, Zhang Yin

*Corresponding author for this work

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

A biosensor based on AuNP modified GC electrodes has been developed for direct detection of methyl parathion. AuNP can be introduced to mixed monolayers of aryldiazonium salt modified GC electrodes by Au-C bonding through aryldiazonium salt chemistry, which provides a stable interface showing efficient electron transfer between biomolecules and electrodes. PEG molecules were introduced to the interface to resist non-specific protein adsorption. AuNP surfaces were further modified with 4-carboxyphenyl followed by covalent immobilization of methyl parathion hydrolase (MPH), a specific biocatalytic enzyme to methyl parathion. Exposure of this interface to methyl parathion resulted in a change in amperometric signal due to the MPH catalytic hydrolysis of methyl parathion producing electroactive compound 4-nitrophenol. This biosensor shows high selectivity, specificity, reproducibility and stability, and is functional for the detection of methyl parathion in real samples. The linear range for detection of methyl parathion is 0.2-100. ppb with a detection limit of 0.07. ppb in 0.1. M phosphate buffer at pH 7.0.

Original languageEnglish
Pages (from-to)440-446
Number of pages7
JournalBiosensors and Bioelectronics
Volume53
DOIs
Publication statusPublished - 15 Mar 2014
Externally publishedYes

Keywords

  • Aryldiazonium salts
  • Au-C bonding
  • AuNP
  • Methyl parathion
  • Methyl parathion hydrolase

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