Covalent functionalization of gold nanoparticles as electronic bridges and signal amplifiers towards an electrochemical immunosensor for botulinum neurotoxin type A

Guozhen Liu*, Yin Zhang, Wenqi Guo

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

39 Citations (Scopus)

Abstract

This work introduced an efficient approach for modification of AuNPs with multicomponents by diazonium salt couplings. The multifunctionalized AuNPs with protruding functional groups that allow simple bioconjugation to large amounts of biomolecules have been successfully used as electronic bridges and signal amplifiers for an electrochemical immunosensor towards the detection of BoNT/A. The one-step anchoring AuNPs strategy has greatly increased the efficiency for attachment of biomolecules and subsequently increased the sensitivity. Sensitivity was further amplified by preparation of bioconjugates particles containing horseradish peroxidase (HRP) labels along with detection antibodies (AbL) attached to AuNPs. The immunosensor can be used for the detection of BoNT/A over the range of 4-35pgmL-1 with the lowest detection limit of 1pgmL-1 and assay time of 10min. The herein sensing strategy is rapid, robust, selective, sensitive, and is promising for future fabrication of point-of-care devices.

Original languageEnglish
Pages (from-to)547-553
Number of pages7
JournalBiosensors and Bioelectronics
Volume61
DOIs
Publication statusPublished - 15 Nov 2014
Externally publishedYes

Keywords

  • Au-C bonding
  • AuNP
  • BoNT/A
  • Immunosensor
  • Signal amplification

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