CPF impedes cell cycle re-entry of quiescent lung cancer cells through transcriptional suppression of FACT and c-MYC

Ling Bi, Chanlu Xie, Lijing Jiao, Shenyi jin, Su Su Thae Hnit, Yao Mu, Yilun Wang, Qian Wang, Guangbo Ge, Yaqiao Wang, Xiaodong Zhao, Xinglong Shi, Yani Kang, Paul De Souza, Tao Liu, Jia Zhou, Ling Xu*, Qihan Dong*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)
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Abstract

Blockade of cell cycle re-entry in quiescent cancer cells is a strategy to prevent cancer progression and recurrence. We investigated the action and mode of action of CPF mixture (Coptis chinensis, Pinellia ternata and Fructus trichosanthis) in impeding a proliferative switch in quiescent lung cancer cells. The results indicated that CPF impeded cell cycle re-entry in quiescent lung cancer cells by reduction of FACT and c-MYC mRNA and protein levels, with concomitant decrease in H3K4 tri-methylation and RNA polymerase II occupancy at FACT and c-MYC promoter regions. Animals implanted with quiescent cancer cells that had been exposed to CPF had reduced tumour volume/weight. Thus, CPF suppresses proliferative switching through transcriptional suppression of FACT and the c-MYC, providing a new insight into therapeutic target and intervention method in impeding cancer recurrence.
Original languageEnglish
Pages (from-to)2229-2239
Number of pages11
JournalJournal of Cellular and Molecular Medicine
Volume24
Issue number3
Early online date20 Jan 2020
DOIs
Publication statusPublished - Feb 2020
Externally publishedYes

Bibliographical note

Copyright the Author(s) 2020. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

Keywords

  • C-MYC
  • G0 cell cycle re-entry
  • lung cancer
  • structure-specific recognition protein 1
  • suppressor of Ty homolog-16
  • c-MYC
  • G cell cycle re-entry

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