TY - JOUR
T1 - CRAFT
T2 - Multimodality confocal skin imaging for early cancer diagnosis
AU - Peng, Tong
AU - Xie, Hao
AU - Ding, Yichen
AU - Wang, Weichao
AU - Li, Zhiming
AU - Jin, Dayong
AU - Tang, Yuanhe
AU - Ren, Qiushi
AU - Xi, Peng
PY - 2012/5
Y1 - 2012/5
N2 - Although histological analysis serves as a gold standard to cancer diagnosis, its application on skin cancer detection is largely prohibited due to its invasive nature. To obtain both the structural and pathological information in situ, a Confocal Reflectance/Auto-Fluorescence Tomography (CRAFT) system was established to examine the skin sites in vivo with both reflectance and autofluorescence modes simultaneously. Nude mice skin with cancerous sites and normal skin sites were imaged and compared with the system. The cellular density and reflective intensity in cancerous sites reflects the structural change of the tissue. With the decay coefficient analysis, the corresponding NAD(P)H decay index for cancerous sites is 1.65-fold that of normal sites, leading to a 97.8% of sensitivity and specificity for early cancer diagnosis. The results are verified by the followed histological analysis. Therefore, CRAFT may provide a novel method for the in vivo, non-invasive diagnosis of early cancer.
AB - Although histological analysis serves as a gold standard to cancer diagnosis, its application on skin cancer detection is largely prohibited due to its invasive nature. To obtain both the structural and pathological information in situ, a Confocal Reflectance/Auto-Fluorescence Tomography (CRAFT) system was established to examine the skin sites in vivo with both reflectance and autofluorescence modes simultaneously. Nude mice skin with cancerous sites and normal skin sites were imaged and compared with the system. The cellular density and reflective intensity in cancerous sites reflects the structural change of the tissue. With the decay coefficient analysis, the corresponding NAD(P)H decay index for cancerous sites is 1.65-fold that of normal sites, leading to a 97.8% of sensitivity and specificity for early cancer diagnosis. The results are verified by the followed histological analysis. Therefore, CRAFT may provide a novel method for the in vivo, non-invasive diagnosis of early cancer.
UR - http://www.scopus.com/inward/record.url?scp=84860598411&partnerID=8YFLogxK
U2 - 10.1002/jbio.201100124
DO - 10.1002/jbio.201100124
M3 - Article
C2 - 22232081
AN - SCOPUS:84860598411
SN - 1864-063X
VL - 5
SP - 469
EP - 476
JO - Journal of Biophotonics
JF - Journal of Biophotonics
IS - 5-6
ER -