Cross-species amplification of microsatellite loci in aphids: assessment and application

Alex Wilson, Blandine Massonnet, Jean-Christophe Simon, Nathalie Prunier-Leterme, Lotfali Dolatti, Kate Llewellyn, Christian Figueroa, Claudio Ramirez, Roger Blackman, Arnaud Estoup, Paul Sunnucks

    Research output: Contribution to journalArticlepeer-review

    119 Citations (Scopus)

    Abstract

    Despite the relative ease of isolating microsatellites, their development still requires substantial inputs of time, money and expertise. For this reason there is considerable interest in using existing microsatellites on species from which markers were not cloned. We tested cross-species amplification of 48 existing aphid loci in species of the following genera: Aphidinae: Aphidini: Aphis and Rhopalosiphum; Aphidinae: Macrosiphini: Acyrthosiphum, Brevicoryne, Diuraphis, Illinoia, Macrosiphoniella, Macrosiphum, Metopeurum, Metapolophium, Myzus, Phorodon, Sitobion and Uroleucon and Neuquenaphidinae: Neuquenaphis. Our results show cross-species application of known microsatellite loci is a highly promising source of codominant markers for population genetic and evolutionary studies in aphids.
    Original languageEnglish
    Pages (from-to)104-109
    Number of pages6
    JournalMolecular Ecology Notes
    Volume4
    Issue number1
    DOIs
    Publication statusPublished - 2004

    Keywords

    • Aphidini
    • Cross-priming
    • Macrosiphini
    • Microsatellite
    • Neuquenaphidinae
    • X-linked

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