Cross-species amplification of microsatellite loci in aphids: assessment and application

Alex Wilson; Blandine Massonnet; Jean-Christophe Simon; Nathalie Prunier-Leterme; Lotfali Dolatti; Kate Llewellyn; Christian Figueroa; Claudio Ramirez; Roger Blackman; Arnaud Estoup; Paul Sunnucks

doi:10.1046/j.1471-8286.2003.00584.x

Cross-species amplification of microsatellite loci in aphids: assessment and application

Alex Wilson, Blandine Massonnet, Jean-Christophe Simon, Nathalie Prunier-Leterme, Lotfali Dolatti, Kate Llewellyn, Christian Figueroa, Claudio Ramirez, Roger Blackman, Arnaud Estoup, Paul Sunnucks

Research output: Contribution to journal › Article › peer-review

123 Citations (Scopus)

Abstract

Despite the relative ease of isolating microsatellites, their development still requires substantial inputs of time, money and expertise. For this reason there is considerable interest in using existing microsatellites on species from which markers were not cloned. We tested cross-species amplification of 48 existing aphid loci in species of the following genera: Aphidinae: Aphidini: Aphis and Rhopalosiphum; Aphidinae: Macrosiphini: Acyrthosiphum, Brevicoryne, Diuraphis, Illinoia, Macrosiphoniella, Macrosiphum, Metopeurum, Metapolophium, Myzus, Phorodon, Sitobion and Uroleucon and Neuquenaphidinae: Neuquenaphis. Our results show cross-species application of known microsatellite loci is a highly promising source of codominant markers for population genetic and evolutionary studies in aphids.

Original language	English
Pages (from-to)	104-109
Number of pages	6
Journal	Molecular Ecology Notes
Volume	4
Issue number	1
DOIs	https://doi.org/10.1046/j.1471-8286.2003.00584.x
Publication status	Published - 2004

Keywords

Aphidini
Cross-priming
Macrosiphini
Microsatellite
Neuquenaphidinae
X-linked

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10.1046/j.1471-8286.2003.00584.x

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title = "Cross-species amplification of microsatellite loci in aphids: assessment and application",

abstract = "Despite the relative ease of isolating microsatellites, their development still requires substantial inputs of time, money and expertise. For this reason there is considerable interest in using existing microsatellites on species from which markers were not cloned. We tested cross-species amplification of 48 existing aphid loci in species of the following genera: Aphidinae: Aphidini: Aphis and Rhopalosiphum; Aphidinae: Macrosiphini: Acyrthosiphum, Brevicoryne, Diuraphis, Illinoia, Macrosiphoniella, Macrosiphum, Metopeurum, Metapolophium, Myzus, Phorodon, Sitobion and Uroleucon and Neuquenaphidinae: Neuquenaphis. Our results show cross-species application of known microsatellite loci is a highly promising source of codominant markers for population genetic and evolutionary studies in aphids.",

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author = "Alex Wilson and Blandine Massonnet and Jean-Christophe Simon and Nathalie Prunier-Leterme and Lotfali Dolatti and Kate Llewellyn and Christian Figueroa and Claudio Ramirez and Roger Blackman and Arnaud Estoup and Paul Sunnucks",

year = "2004",

doi = "10.1046/j.1471-8286.2003.00584.x",

language = "English",

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pages = "104--109",

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T1 - Cross-species amplification of microsatellite loci in aphids

T2 - assessment and application

AU - Wilson, Alex

AU - Massonnet, Blandine

AU - Simon, Jean-Christophe

AU - Prunier-Leterme, Nathalie

AU - Dolatti, Lotfali

AU - Llewellyn, Kate

AU - Figueroa, Christian

AU - Ramirez, Claudio

AU - Blackman, Roger

AU - Estoup, Arnaud

AU - Sunnucks, Paul

PY - 2004

Y1 - 2004

N2 - Despite the relative ease of isolating microsatellites, their development still requires substantial inputs of time, money and expertise. For this reason there is considerable interest in using existing microsatellites on species from which markers were not cloned. We tested cross-species amplification of 48 existing aphid loci in species of the following genera: Aphidinae: Aphidini: Aphis and Rhopalosiphum; Aphidinae: Macrosiphini: Acyrthosiphum, Brevicoryne, Diuraphis, Illinoia, Macrosiphoniella, Macrosiphum, Metopeurum, Metapolophium, Myzus, Phorodon, Sitobion and Uroleucon and Neuquenaphidinae: Neuquenaphis. Our results show cross-species application of known microsatellite loci is a highly promising source of codominant markers for population genetic and evolutionary studies in aphids.

AB - Despite the relative ease of isolating microsatellites, their development still requires substantial inputs of time, money and expertise. For this reason there is considerable interest in using existing microsatellites on species from which markers were not cloned. We tested cross-species amplification of 48 existing aphid loci in species of the following genera: Aphidinae: Aphidini: Aphis and Rhopalosiphum; Aphidinae: Macrosiphini: Acyrthosiphum, Brevicoryne, Diuraphis, Illinoia, Macrosiphoniella, Macrosiphum, Metopeurum, Metapolophium, Myzus, Phorodon, Sitobion and Uroleucon and Neuquenaphidinae: Neuquenaphis. Our results show cross-species application of known microsatellite loci is a highly promising source of codominant markers for population genetic and evolutionary studies in aphids.

KW - Aphidini

KW - Cross-priming

KW - Macrosiphini

KW - Microsatellite

KW - Neuquenaphidinae

KW - X-linked

U2 - 10.1046/j.1471-8286.2003.00584.x

DO - 10.1046/j.1471-8286.2003.00584.x

M3 - Article

SN - 1471-8278

VL - 4

SP - 104

EP - 109

JO - Molecular Ecology Notes

JF - Molecular Ecology Notes

IS - 1

ER -

Cross-species amplification of microsatellite loci in aphids: assessment and application

Abstract

Keywords

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