Cross Species applicability of Anti-human plasma IgY affinity column

Abidali Mohamedali, Sock Tan, Sadia Mahboob, Amit Kapur, M.S. Baker

Research output: Contribution to journalArticlepeer-review

36 Downloads (Pure)

Abstract

In order to improve the sensitivity of proteomic analysis, our group has developed a chicken antibody based column that has successfully depleted high abundance proteins (HAPs) from human plasma prior to mass spectrometry analysis. The use of chicken as a host for polyclonal antibodies production conveys many advantages, including but not limited to, the capabilities of producing large quantities of IgY antibodies with minimal antigen quantities and enhanced immunogenicity exhibited by their immune systems against foreign immunogens. In this study, we explored the ability to use an anti-human IgY affinity column with a variety of plasma samples derived from different animals and demonstrate this column’s ability to deplete the two most common HAPs (albumin and immunoglobulins) reproducibly in phylogenetically related species. Hence, our study highly recommends that IgY-based immune depletion technologies could be used to capture targeted proteins among phylogenetically related species without having to produce separate columns for each clade.
Original languageEnglish
Article number779
Pages (from-to)1-5
Number of pages5
JournalJournal of Immunology and Serum Biology
Volume2
Issue number1
DOIs
Publication statusPublished - 11 Jun 2016

Bibliographical note

Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

Keywords

  • Ultradepletion
  • Animal plasma
  • API
  • IgY
  • Chicken antibodies
  • Phylogenetic relation

Fingerprint Dive into the research topics of 'Cross Species applicability of Anti-human plasma IgY affinity column'. Together they form a unique fingerprint.

Cite this