Crossing streamlines to lyse cells and simultaneously separate genomic contents

Keith J. Morton, Kevin Loutherback, David W. Inglis, James C. Sturm, Robert H. Austin, Stephen Y. Chou

Research output: Chapter in Book/Report/Conference proceedingConference proceeding contribution

Abstract

We show that it is possible to direct cells across streamlines and into adjacent, functional flows enabling on-chip lysis and genomic DNA extraction. We use an asymmetric post array (bump array) [1] to move cells across flows having different chemical constituents. We demonstrate that spheroplasted E.coli cells can be transported into a parallel, co-flowing lysis stream where the cell is lysed and the cell contents are extracted; following lysis the genomic DNA is immediately and simultaneously separated from smaller cellular debris.

Original languageEnglish
Title of host publication12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
Subtitle of host publicationProceedings
Place of PublicationWashington, DC
PublisherChemical and Biological Microsystems Society
Pages1965-1967
Number of pages3
Publication statusPublished - 2008
Externally publishedYes
Event12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States
Duration: 12 Oct 200816 Oct 2008

Other

Other12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
CountryUnited States
CitySan Diego, CA
Period12/10/0816/10/08

Keywords

  • Cell lysis
  • Cell separation
  • Genomics
  • Sorting

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