Crossing streamlines to lyse cells and simultaneously separate genomic contents

Keith J. Morton; Kevin Loutherback; David W. Inglis; James C. Sturm; Robert H. Austin; Stephen Y. Chou

Crossing streamlines to lyse cells and simultaneously separate genomic contents

Keith J. Morton, Kevin Loutherback, David W. Inglis, James C. Sturm, Robert H. Austin, Stephen Y. Chou

Research output: Chapter in Book/Report/Conference proceeding › Conference proceeding contribution › peer-review

Abstract

We show that it is possible to direct cells across streamlines and into adjacent, functional flows enabling on-chip lysis and genomic DNA extraction. We use an asymmetric post array (bump array) [1] to move cells across flows having different chemical constituents. We demonstrate that spheroplasted E.coli cells can be transported into a parallel, co-flowing lysis stream where the cell is lysed and the cell contents are extracted; following lysis the genomic DNA is immediately and simultaneously separated from smaller cellular debris.

Original language	English
Title of host publication	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
Subtitle of host publication	Proceedings
Place of Publication	Washington, DC
Publisher	Chemical and Biological Microsystems Society
Pages	1965-1967
Number of pages	3
Publication status	Published - 2008
Externally published	Yes
Event	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States Duration: 12 Oct 2008 → 16 Oct 2008

Other

Other	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
Country/Territory	United States
City	San Diego, CA
Period	12/10/08 → 16/10/08

Keywords

Cell lysis
Cell separation
Genomics
Sorting

Cite this

@inproceedings{69d0acc709964c94ae29f032e2c51de0,

title = "Crossing streamlines to lyse cells and simultaneously separate genomic contents",

abstract = "We show that it is possible to direct cells across streamlines and into adjacent, functional flows enabling on-chip lysis and genomic DNA extraction. We use an asymmetric post array (bump array) [1] to move cells across flows having different chemical constituents. We demonstrate that spheroplasted E.coli cells can be transported into a parallel, co-flowing lysis stream where the cell is lysed and the cell contents are extracted; following lysis the genomic DNA is immediately and simultaneously separated from smaller cellular debris.",

keywords = "Cell lysis, Cell separation, Genomics, Sorting",

author = "Morton, \{Keith J.\} and Kevin Loutherback and Inglis, \{David W.\} and Sturm, \{James C.\} and Austin, \{Robert H.\} and Chou, \{Stephen Y.\}",

year = "2008",

language = "English",

pages = "1965--1967",

booktitle = "12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008",

publisher = "Chemical and Biological Microsystems Society",

note = "12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 ; Conference date: 12-10-2008 Through 16-10-2008",

}

Morton, KJ, Loutherback, K, Inglis, DW, Sturm, JC, Austin, RH & Chou, SY 2008, Crossing streamlines to lyse cells and simultaneously separate genomic contents. in 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008: Proceedings. Chemical and Biological Microsystems Society, Washington, DC, pp. 1965-1967, 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, San Diego, CA, United States, 12/10/08.

Crossing streamlines to lyse cells and simultaneously separate genomic contents. / Morton, Keith J.; Loutherback, Kevin; Inglis, David W. et al.
12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008: Proceedings. Washington, DC: Chemical and Biological Microsystems Society, 2008. p. 1965-1967.

Research output: Chapter in Book/Report/Conference proceeding › Conference proceeding contribution › peer-review

TY - GEN

T1 - Crossing streamlines to lyse cells and simultaneously separate genomic contents

AU - Morton, Keith J.

AU - Loutherback, Kevin

AU - Inglis, David W.

AU - Sturm, James C.

AU - Austin, Robert H.

AU - Chou, Stephen Y.

PY - 2008

Y1 - 2008

N2 - We show that it is possible to direct cells across streamlines and into adjacent, functional flows enabling on-chip lysis and genomic DNA extraction. We use an asymmetric post array (bump array) [1] to move cells across flows having different chemical constituents. We demonstrate that spheroplasted E.coli cells can be transported into a parallel, co-flowing lysis stream where the cell is lysed and the cell contents are extracted; following lysis the genomic DNA is immediately and simultaneously separated from smaller cellular debris.

AB - We show that it is possible to direct cells across streamlines and into adjacent, functional flows enabling on-chip lysis and genomic DNA extraction. We use an asymmetric post array (bump array) [1] to move cells across flows having different chemical constituents. We demonstrate that spheroplasted E.coli cells can be transported into a parallel, co-flowing lysis stream where the cell is lysed and the cell contents are extracted; following lysis the genomic DNA is immediately and simultaneously separated from smaller cellular debris.

KW - Cell lysis

KW - Cell separation

KW - Genomics

KW - Sorting

UR - https://www.scopus.com/pages/publications/84902440707

M3 - Conference proceeding contribution

AN - SCOPUS:84902440707

SP - 1965

EP - 1967

BT - 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008

PB - Chemical and Biological Microsystems Society

CY - Washington, DC

T2 - 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008

Y2 - 12 October 2008 through 16 October 2008

ER -

Crossing streamlines to lyse cells and simultaneously separate genomic contents

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Keywords

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