Crystallization and preliminary X-ray analysis of the Rhodobacter capsulatus magnesium chelatase BchI subunit

Robert D. Willows*, Mats Hansson, Samuel I. Beale, Martin Laurberg, Salam Al-Karadaghi

*Corresponding author for this work

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The Rhodobacter capsulatus BchI protein is one of three subunits of Mg chelatase, the enzyme which catalyzes the first committed step of chlorophyll and bacteriochlorophyll biosynthesis. The BchI protein was produced with an inducible T7 RNA polymerase expression system in Escherichia coli. The protein was purified from the soluble cell-extract fraction and crystallized from polyethylene glycol solution. The crystals diffract to a minimum Bragg spacing of 2.1 Å. The space group is P63 with unit-cell dimensions a = b = 90.6, c = 84.1 Å.

Original languageEnglish
Pages (from-to)689-690
Number of pages2
JournalActa Crystallographica Section D: Biological Crystallography
Volume55
Issue number3
DOIs
Publication statusPublished - Mar 1999
Externally publishedYes

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