The allophanate hydrolase from Pseudomonas sp. strain ADP was expressed and purified, and a tryptic digest fragment was subsequently identified, expressed and purified. This 50 kDa construct retained amidase activity and was crystallized. The crystals diffracted to 2.5 Å resolution and adopted space group P21, with unit-cell parameters a = 82.4, b = 179.2, c = 112.6 Å, β = 106.6°.
|Number of pages||6|
|Journal||Acta Crystallographica. Section F: Structural Biology Communications|
|Publication status||Published - 2014|
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- allphanate hydrolase
- amidase domain
- Pseudomonas sp. strain ADP