Cultivation of fastidious bacteria by viability staining and micromanipulation in a soil substrate membrane system

B. C. Ferrari, M. R. Gillings

    Research output: Contribution to journalArticlepeer-review

    31 Citations (Scopus)

    Abstract

    Soil substrate membrane systems allow for microcultivation of fastidious soil bacteria as mixed microbial communities. We isolated established microcolonies from these membranes by using fluorescence viability staining and micromanipulation. This approach facilitated the recovery of diverse, novel isolates, including the recalcitrant bacterium Leifsonia xyli, a plant pathogen that has never been isolated outside the host.

    Original languageEnglish
    Pages (from-to)3352-3354
    Number of pages3
    JournalApplied and Environmental Microbiology
    Volume75
    Issue number10
    DOIs
    Publication statusPublished - May 2009

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