Cultivation of fastidious bacteria by viability staining and micromanipulation in a soil substrate membrane system

B. C. Ferrari; M. R. Gillings

doi:10.1128/AEM.02407-08

Cultivation of fastidious bacteria by viability staining and micromanipulation in a soil substrate membrane system

B. C. Ferrari, M. R. Gillings

Research output: Contribution to journal › Article › peer-review

31 Citations (Scopus)

Abstract

Soil substrate membrane systems allow for microcultivation of fastidious soil bacteria as mixed microbial communities. We isolated established microcolonies from these membranes by using fluorescence viability staining and micromanipulation. This approach facilitated the recovery of diverse, novel isolates, including the recalcitrant bacterium Leifsonia xyli, a plant pathogen that has never been isolated outside the host.

Original language	English
Pages (from-to)	3352-3354
Number of pages	3
Journal	Applied and Environmental Microbiology
Volume	75
Issue number	10
DOIs	https://doi.org/10.1128/AEM.02407-08
Publication status	Published - May 2009

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title = "Cultivation of fastidious bacteria by viability staining and micromanipulation in a soil substrate membrane system",

abstract = "Soil substrate membrane systems allow for microcultivation of fastidious soil bacteria as mixed microbial communities. We isolated established microcolonies from these membranes by using fluorescence viability staining and micromanipulation. This approach facilitated the recovery of diverse, novel isolates, including the recalcitrant bacterium Leifsonia xyli, a plant pathogen that has never been isolated outside the host.",

author = "Ferrari, {B. C.} and Gillings, {M. R.}",

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AU - Gillings, M. R.

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N2 - Soil substrate membrane systems allow for microcultivation of fastidious soil bacteria as mixed microbial communities. We isolated established microcolonies from these membranes by using fluorescence viability staining and micromanipulation. This approach facilitated the recovery of diverse, novel isolates, including the recalcitrant bacterium Leifsonia xyli, a plant pathogen that has never been isolated outside the host.

AB - Soil substrate membrane systems allow for microcultivation of fastidious soil bacteria as mixed microbial communities. We isolated established microcolonies from these membranes by using fluorescence viability staining and micromanipulation. This approach facilitated the recovery of diverse, novel isolates, including the recalcitrant bacterium Leifsonia xyli, a plant pathogen that has never been isolated outside the host.

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AN - SCOPUS:66249126846

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JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

IS - 10

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Cultivation of fastidious bacteria by viability staining and micromanipulation in a soil substrate membrane system

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