Cultivation of fastidious bacteria by viability staining and micromanipulation in a soil substrate membrane system

B. C. Ferrari, M. R. Gillings

Research output: Contribution to journalArticlepeer-review

29 Citations (Scopus)

Abstract

Soil substrate membrane systems allow for microcultivation of fastidious soil bacteria as mixed microbial communities. We isolated established microcolonies from these membranes by using fluorescence viability staining and micromanipulation. This approach facilitated the recovery of diverse, novel isolates, including the recalcitrant bacterium Leifsonia xyli, a plant pathogen that has never been isolated outside the host.

Original languageEnglish
Pages (from-to)3352-3354
Number of pages3
JournalApplied and Environmental Microbiology
Volume75
Issue number10
DOIs
Publication statusPublished - May 2009

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