Abstract
Soil substrate membrane systems allow for microcultivation of fastidious soil bacteria as mixed microbial communities. We isolated established microcolonies from these membranes by using fluorescence viability staining and micromanipulation. This approach facilitated the recovery of diverse, novel isolates, including the recalcitrant bacterium Leifsonia xyli, a plant pathogen that has never been isolated outside the host.
| Original language | English |
|---|---|
| Pages (from-to) | 3352-3354 |
| Number of pages | 3 |
| Journal | Applied and Environmental Microbiology |
| Volume | 75 |
| Issue number | 10 |
| DOIs | |
| Publication status | Published - May 2009 |
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