Culture and characterisation of peripheral blood monocytes and monocyte-derived adherent cells of the tammar wallaby, Macropus eugenii

L. J. Young, E. M. Deane

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Monocytes, monocyte-derived adherent cells and dendritic cells all play a role in cellular immunity. In this study, we describe the isolation of monocyte-derived adherent cells and dendritic cells from a model marsupial, the tammar wallaby, Macropus eugenii, and report that in vitro, these cells appear morphologically similar to these cells found in other mammals. The successful culture of marsupial monocyte and dendritic cells was undertaken in serum-free medium which contained lymphocyte conditioned medium as an absolute requirement. This supports the view that similar to cultured dendritic cells from other species reported to date, specific growth factors are required to promote the maturation and differentiation of these cells.

Original languageEnglish
Pages (from-to)253-259
Number of pages7
JournalImmunology Letters
Volume96
Issue number2
DOIs
Publication statusPublished - 31 Jan 2005

Keywords

  • Dendritic cells
  • Marsupials
  • Monocytes
  • Tammar wallaby

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