Culture and stimulation of tammar wallaby lymphocytes

L. J. Young*, E. M. Deane

*Corresponding author for this work

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

We describe the culture and stimulation of lymphocytes from the model marsupial, the tammar wallaby (Macropus eugenii). We also describe the capacity of tammar wallaby lymphocytes isolated from blood, spleen and lymph nodes to produce soluble immunomodulatory factors. Culture conditions were optimized for mitogen-driven stimulation using the plant lectin phytohaemagglutinin (PHA). Products secreted by stimulated cells were harvested and crudely fractionated before they were added back to freshly isolated lymphocytes. Using the 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT) assay, both stimulatory and inhibitory bioactive factors were detected in serum-free supernatants harvested from mitogen-treated peripheral blood mononuclear cells. This paper describes the capacity of leukocytes of the tammar wallaby to respond to mitogenic stimulation and to produce soluble, low-molecular-weight bioactive molecules that possess cytokine-like activity.

Original languageEnglish
Pages (from-to)685-701
Number of pages17
JournalVeterinary Research Communications
Volume31
Issue number6
DOIs
Publication statusPublished - Aug 2007

Keywords

  • Bioactivity
  • Macropus eugenii
  • Marsupial
  • Mitogen
  • PBMC
  • Serum-free
  • Stimulation
  • Tammar wallaby

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