Cytokinin biochemistry in relation to leaf senescence I. The metabolism of 6-benzylaminopurine and zeatin in oat leaf segments

G. Q. Tao*, D. S. Letham, L. M S Palni, R. E. Summons

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    24 Citations (Scopus)

    Abstract

    The metabolism of zeatin and that of 6-benzylaminopurine (BAP) have been compared in oat leaf segments in relation to the markedly differing ability of these cytokinins to retard senescence of such segments. Free BAP and a highly active senescence-retarding metabolite of BAP were detected in oat leaf segments supplied with BAP. The metabolite was identified by mass spectrometry and chromatography as 3-β-D-glucopyranosyl-BAP. The major metabolite of BAP was the 9-glucoside, but this lacked significant senescence-retarding activity. In contrast, in leaf segments supplied with zeatin, no free zeatin and no senescence-retarding metabolite of zeatin were detectable. The major metabolites of zeatin were adenosine, adenine nucleotides, the 9-glucoside, and unidentified polar metabolites. The differing activities of zeatin and BAP in the oat-leaf senescence bioassay appear to be, at least partially, a consequence of their differing metabolism and are not attributable to differences in uptake.

    Original languageEnglish
    Pages (from-to)89-102
    Number of pages14
    JournalJournal of Plant Growth Regulation
    Volume2
    Issue number1-3
    DOIs
    Publication statusPublished - Aug 1983

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