Cytokinins of dry Zea mays seed: Quantification by radioimmunoassay and gas chromatography-mass spectrometry

C. H. Hocart*, J. Badenoch-Jones, C. W. Parker, D. S. Letham, R. E. Summons

*Corresponding author for this work

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    18 Citations (Scopus)


    The endogenous cytokinins present in dry Zea mays seed were determined using both radioimmunoassay and gas chromatography-mass spectrometry. Similar values for bases and ribosides were obtained by the two methods. The cytokinins present in embryo and endosperm were estimated separately using radioimmunoassay; similar levels of cytokinins were found in these two tissues. The major cytokinins detected on a whole-seed basis were dihydrozeatin riboside, O-glucosyldihydrozeatin riboside, zeatin 9-glucoside, zeatin, and the nucleotides of zeatin, dihydrozeatin, and isopentenyladenine. Cytokinin levels in the mature dry seed were considerably lower than cytokinin levels published in the literature for immature seed. Unexpected activity in the radioimmunoassays was detected in the wash from the DEAE cellulose column chromatography step. The compound(s) responsible for this activity did not have the solvent partitioning characteristics of a cytokinin base or riboside. They eluted as a single fraction following high-performance liquid chromatography on a Zorbax C8 column; this fraction showed no activity in the Amaranthus bioassay for cytokinins, but inhibited the activity of authentic zeatin riboside present at an optimal concentration.

    Original languageEnglish
    Pages (from-to)179-196
    Number of pages18
    JournalJournal of Plant Growth Regulation
    Issue number3
    Publication statusPublished - Sep 1988


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