TY - GEN
T1 - Cytotoxicity of single-walled carbon nanotubes with human ocular cells
AU - Yan, Lu
AU - Zhang, Shu
AU - Zeng, Chao
AU - Xue, Yuhua
AU - Zhou, Zhonglou
AU - Lu, Fan
AU - Chen, Hao
AU - Qu, Jia
AU - Dai, Liming
AU - Liu, Yong
PY - 2011
Y1 - 2011
N2 - In this paper, we report the first study on cytotoxicity of single-walled carbon nanotubes (SWCNTs) and theirs derivatives with human ocular cells, such as ARPE-19 cells. In particular, we have systematically investigated the cytotoxicity of SWCNTs, hydroxyl-functionalized SWCNTs (SWCNT-OH), and carboxylic functionalized SWCNTs (SWCNT-COOH) with ARPE-19 cells by examining their influence on the cell morphology, viability, oxidative stress, membrane integrity and apoptosis. To this end, various methods, including optical micrography, CCK-8 assay, LDH assay, SOD assay, TEM and Apoptosis assay, have been used in this study. Our results suggest that SWCNTs could cause an decrease in the cell survival rate, changes in the SOD level, membrane integrity and cell apoptosis, indicating a high toxicity to ARPE-19 cells. However, chemical functionalization of SWCNTs with -OH and -COOH groups was found to significantly improve the biocompatibility of SWCNTs. Among the SWCNTs and their derivatives studied in this work, the SWCNT-COOH exhibits the best biocompatibility to ARPE-19 cells.
AB - In this paper, we report the first study on cytotoxicity of single-walled carbon nanotubes (SWCNTs) and theirs derivatives with human ocular cells, such as ARPE-19 cells. In particular, we have systematically investigated the cytotoxicity of SWCNTs, hydroxyl-functionalized SWCNTs (SWCNT-OH), and carboxylic functionalized SWCNTs (SWCNT-COOH) with ARPE-19 cells by examining their influence on the cell morphology, viability, oxidative stress, membrane integrity and apoptosis. To this end, various methods, including optical micrography, CCK-8 assay, LDH assay, SOD assay, TEM and Apoptosis assay, have been used in this study. Our results suggest that SWCNTs could cause an decrease in the cell survival rate, changes in the SOD level, membrane integrity and cell apoptosis, indicating a high toxicity to ARPE-19 cells. However, chemical functionalization of SWCNTs with -OH and -COOH groups was found to significantly improve the biocompatibility of SWCNTs. Among the SWCNTs and their derivatives studied in this work, the SWCNT-COOH exhibits the best biocompatibility to ARPE-19 cells.
KW - Apoptosis assay
KW - CCK-8 assay
KW - Human RPE cells
KW - LDH assay
KW - Ocular biocompatibility
KW - Single-walled carbon nanotubes (SWCNTs)
KW - SOD assay
UR - http://www.scopus.com/inward/record.url?scp=79961231454&partnerID=8YFLogxK
U2 - 10.4028/www.scientific.net/AMR.287-290.32
DO - 10.4028/www.scientific.net/AMR.287-290.32
M3 - Conference proceeding contribution
AN - SCOPUS:79961231454
SN - 9783037851920
VL - 287-290
T3 - Advanced Materials Research
SP - 32
EP - 36
BT - Applications of Engineering Materials
A2 - Bu, Jinglong
A2 - Wang, Pengcheng
A2 - Ai, Liqun
A2 - Sang, Xiaoming
A2 - Li, Yungang
PB - Trans Tech Publications
CY - Stafa-Zurich, Switzerland
T2 - 2011 International Conference on Advanced Engineering Materials and Technology, AEMT 2011
Y2 - 29 July 2011 through 31 July 2011
ER -