Cytotoxicity of single-walled carbon nanotubes with human ocular cells

Lu Yan*, Shu Zhang, Chao Zeng, Yuhua Xue, Zhonglou Zhou, Fan Lu, Hao Chen, Jia Qu, Liming Dai, Yong Liu

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference proceeding contribution

5 Citations (Scopus)

Abstract

In this paper, we report the first study on cytotoxicity of single-walled carbon nanotubes (SWCNTs) and theirs derivatives with human ocular cells, such as ARPE-19 cells. In particular, we have systematically investigated the cytotoxicity of SWCNTs, hydroxyl-functionalized SWCNTs (SWCNT-OH), and carboxylic functionalized SWCNTs (SWCNT-COOH) with ARPE-19 cells by examining their influence on the cell morphology, viability, oxidative stress, membrane integrity and apoptosis. To this end, various methods, including optical micrography, CCK-8 assay, LDH assay, SOD assay, TEM and Apoptosis assay, have been used in this study. Our results suggest that SWCNTs could cause an decrease in the cell survival rate, changes in the SOD level, membrane integrity and cell apoptosis, indicating a high toxicity to ARPE-19 cells. However, chemical functionalization of SWCNTs with -OH and -COOH groups was found to significantly improve the biocompatibility of SWCNTs. Among the SWCNTs and their derivatives studied in this work, the SWCNT-COOH exhibits the best biocompatibility to ARPE-19 cells.

Original languageEnglish
Title of host publicationApplications of Engineering Materials
EditorsJinglong Bu, Pengcheng Wang, Liqun Ai, Xiaoming Sang, Yungang Li
Place of PublicationStafa-Zurich, Switzerland
PublisherTrans Tech Publications
Pages32-36
Number of pages5
Volume287-290
ISBN (Print)9783037851920
DOIs
Publication statusPublished - 2011
Event2011 International Conference on Advanced Engineering Materials and Technology, AEMT 2011 - Sanya, China
Duration: 29 Jul 201131 Jul 2011

Publication series

NameAdvanced Materials Research
PublisherTrans Tech Publications
Volume287-290
ISSN (Print)1022-6680

Other

Other2011 International Conference on Advanced Engineering Materials and Technology, AEMT 2011
CountryChina
CitySanya
Period29/07/1131/07/11

Keywords

  • Apoptosis assay
  • CCK-8 assay
  • Human RPE cells
  • LDH assay
  • Ocular biocompatibility
  • Single-walled carbon nanotubes (SWCNTs)
  • SOD assay

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