Data visualization of CRISPR-Cas9 guide RNA design tools

Yatish Jain, Fathima Afra Mohamed Izzath, Laurence O. W. Wilson, Denis C. Bauer*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference proceeding contributionpeer-review

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Abstract

With the advancement of genomic engineering and genetic modification techniques, the uptake of computational tools to design guide RNA increased drastically. Searching for genomic targets to design guides with maximum on-target activity (efficiency) and minimum off-target activity (specificity) is now an essential part of genome editing experiments. Today, a variety of tools exist that allow the search of genomic targets and let users customize their search parameters to better suit their experiments. Here we present an overview of different ways to visualize these searched CRISPR target sites along with specific downstream information like primer design, restriction enzyme activity and mutational outcome prediction after a double-stranded break. We discuss the importance of a good visualization summary to interpret information along with different ways to represent similar information effectively.

Original languageEnglish
Title of host publicationMEDINFO 2023 - The Future is Accessible
Subtitle of host publicationProceedings of the 19th World Congress on Medical and Health Informatics
EditorsJen Bichel-Findlay, Paula Otero, Philip Scott, Elaine Huesing
Place of PublicationAmsterdam
PublisherIOS Press
Pages770-774
Number of pages5
ISBN (Electronic)9781643684574
ISBN (Print)9781643684567
DOIs
Publication statusPublished - 25 Jan 2024
Event19th World Congress on Medical and Health Informatics, MedInfo 2023 - Sydney, Australia
Duration: 8 Jul 202312 Jul 2023

Publication series

NameStudies in Health Technology and Informatics
PublisherIOS Press
Volume310
ISSN (Print)0926-9630
ISSN (Electronic)1879-8365

Conference

Conference19th World Congress on Medical and Health Informatics, MedInfo 2023
Country/TerritoryAustralia
CitySydney
Period8/07/2312/07/23

Bibliographical note

Copyright 2024 International Medical Informatics Association (IMIA) and IOS Press. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

Keywords

  • CRISPR-Cas9
  • design tools
  • gRNA
  • knock-out
  • UX design
  • Visualization

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