TY - JOUR
T1 - De-novo assembly and analysis of the heterozygous triploid genome of the wine spoilage yeast dekkera bruxellensis AWRI1499
AU - Curtin, Chris D.
AU - Borneman, Anthony R.
AU - Chambers, Paul J.
AU - Pretorius, Isak S.
N1 - Copyright the Author(s) [2012]. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.
PY - 2012/3/28
Y1 - 2012/3/28
N2 - Despite its industrial importance, the yeast species Dekkera (Brettanomyces) bruxellensis has remained poorly understood at the genetic level. In this study we describe whole genome sequencing and analysis for a prevalent wine spoilage strain, AWRI1499. The 12.7 Mb assembly, consisting of 324 contigs in 99 scaffolds (super-contigs) at 26-fold coverage, exhibits a relatively high density of single nucleotide polymorphisms (SNPs). Haplotype sampling for 1.2% of open reading frames suggested that the D. bruxellensis AWRI1499 genome is comprised of a moderately heterozygous diploid genome, in combination with a divergent haploid genome. Gene content analysis revealed enrichment in membrane proteins, particularly transporters, along with oxidoreductase enzymes. Availability of this assembly and annotation provides a resource for further investigation of genomic organization in this species, and functional characterization of genes that may confer important phenotypic traits.
AB - Despite its industrial importance, the yeast species Dekkera (Brettanomyces) bruxellensis has remained poorly understood at the genetic level. In this study we describe whole genome sequencing and analysis for a prevalent wine spoilage strain, AWRI1499. The 12.7 Mb assembly, consisting of 324 contigs in 99 scaffolds (super-contigs) at 26-fold coverage, exhibits a relatively high density of single nucleotide polymorphisms (SNPs). Haplotype sampling for 1.2% of open reading frames suggested that the D. bruxellensis AWRI1499 genome is comprised of a moderately heterozygous diploid genome, in combination with a divergent haploid genome. Gene content analysis revealed enrichment in membrane proteins, particularly transporters, along with oxidoreductase enzymes. Availability of this assembly and annotation provides a resource for further investigation of genomic organization in this species, and functional characterization of genes that may confer important phenotypic traits.
UR - http://www.scopus.com/inward/record.url?scp=84859021892&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0033840
DO - 10.1371/journal.pone.0033840
M3 - Article
C2 - 22470482
AN - SCOPUS:84859021892
VL - 7
SP - 1
EP - 10
JO - PLoS ONE
JF - PLoS ONE
SN - 1932-6203
IS - 3
M1 - e33840
ER -