De-novo assembly and analysis of the heterozygous triploid genome of the wine spoilage yeast dekkera bruxellensis AWRI1499

Chris D. Curtin, Anthony R. Borneman, Paul J. Chambers, Isak S. Pretorius

Research output: Contribution to journalArticle

80 Citations (Scopus)
14 Downloads (Pure)

Abstract

Despite its industrial importance, the yeast species Dekkera (Brettanomyces) bruxellensis has remained poorly understood at the genetic level. In this study we describe whole genome sequencing and analysis for a prevalent wine spoilage strain, AWRI1499. The 12.7 Mb assembly, consisting of 324 contigs in 99 scaffolds (super-contigs) at 26-fold coverage, exhibits a relatively high density of single nucleotide polymorphisms (SNPs). Haplotype sampling for 1.2% of open reading frames suggested that the D. bruxellensis AWRI1499 genome is comprised of a moderately heterozygous diploid genome, in combination with a divergent haploid genome. Gene content analysis revealed enrichment in membrane proteins, particularly transporters, along with oxidoreductase enzymes. Availability of this assembly and annotation provides a resource for further investigation of genomic organization in this species, and functional characterization of genes that may confer important phenotypic traits.

Original languageEnglish
Article numbere33840
Pages (from-to)1-10
Number of pages10
JournalPLoS ONE
Volume7
Issue number3
DOIs
Publication statusPublished - 28 Mar 2012
Externally publishedYes

    Fingerprint

Bibliographical note

Copyright the Author(s) [2012]. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

Cite this