Detection systems with deferred registration of luminescence signals are promising for performing complex tasks of imaging of biological objects due to their simplicity and low cost. In the present work, -NaYF4:Tm3+Yb3+/NaYF4 nanocrystals with anti-Stokes photoluminescence have been used in deferred registration systems. It has been shown that there is a significant time delay between the exciting laser pulse and luminescence signal, which makes it possible to use this class of nanoparticles in the creation of wide-field imaging systems with deferred registration. The possibility of using nanoparticles for detecting a photoluminescence signal in the second transparency window of biotissue has been demonstrated. This system can be based on the resonance excitation and detection of the photoluminescence signal of Yb3+ ions.