Defining viral protein interactomes using the yeast two-hybrid assay

Eve Diefenbach, Anthony L. Cunningham, Russell J. Diefenbach*

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

1 Citation (Scopus)

Abstract

The yeast two-hybrid assay has proved a powerful tool in identifying and characterising binary protein-protein interactions. Not only can it be used to map interacting protein domains, it can also be used to screen cDNA libraries with a desired bait to identify novel binding partners. A number of factors including ease of use, cost effectiveness and suitability for high throughput analysis have made yeast-two hybrid one of the assays of choice for defining protein-protein interaction networks or interactomes for a range of organisms. The focus of this review is on the definition of viral interactomes using the yeast two-hybrid assay and the relevance of such studies to our understanding of viral pathogenesis.

Original languageEnglish
Pages (from-to)225-231
Number of pages7
JournalCurrent Proteomics
Volume2
Issue number3
DOIs
Publication statusPublished - Oct 2005
Externally publishedYes

Keywords

  • Drug targets
  • Interactome
  • Proteomics
  • Virus
  • Yeast two-hybrid assay

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