Degenerate oligonucleotide gene shuffling

Moreland David Gibbs; Peter Leonard Bergquist; Kaisu Milja Helena Nevalainen

Degenerate oligonucleotide gene shuffling

Moreland David Gibbs (Inventor), Peter Leonard Bergquist (Inventor), Kaisu Milja Helena Nevalainen (Inventor)

Research output: Patent

Abstract

A method for gene shuffling to form a mutant or chimeric gene, the method conmprising: (a) assigning one or more segments of one or more genes based on regions of encoded amino acid sequence; (b) amplifying the one or more assigned segments of the gene using primers specific for each segment; and (c) causing recombination of the one or more amplified segments to form a mutant or chimeric gene. An oligonucleotide primer suitable for use in gene shuffling, the primer having a non-degenerate core based on a segment or template of a gene to be amplified, and the core being flanked by both 5' and 3' degenerate ends.

Original language	English
Patent number	WO2002018629
IPC	C12N 15/10 2006.01,C12Q 1/68 2006.01
Priority date	29/08/00
Filing date	29/08/01
Publication status	Submitted - 7 Mar 2002

Bibliographical note

Designated States:
AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DZ, EC, EE, ES, FI, GB, GD, GE, GH, GM, HR, HU, ID, IL, IN, IS, JP, KE, KG, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, MA, MD, MG, MK, MN, MW, MX, MZ, NO, NZ, PH, PL, PT, RO, RU, SD, SE, SG, SI, SK, SL, TJ, TM, TR, TT, TZ, UA, UG, US, UZ, VN, YU, ZA, ZW
African Regional Intellectual Property Organization (ARIPO) (GH, GM, KE, LS, MW, MZ, SD, SL, SZ, TZ, UG, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, CH, CY, DE, DK, ES, FI, FR, GB, GR, IE, IT, LU, MC, NL, PT, SE, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG)

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https://patentscope.wipo.int/search/en/detail.jsf?docId=WO2002018629

2 Patent

Degenerate oligonucleotide gene shuffling
Bergquist, P. L., Gibbs, M. D. & Nevalainen, H. K. M., 4 Nov 2004, IPC No. C12N 15/09, C12N 15/10, C12N 15/11, C12Q 1/68, Patent No. AU2001283710, Priority date 29 Aug 2000, Priority No. AUPQ9749
Research output: Patent

Open Access
Degenerate oligonucleotide gene shuffling
Gibbs, M. D., Bergquist, P. L. & Nevalainen, H. K. M., 25 Jun 2003, (Submitted) IPC No. C12N 15/09, C12Q 1/68, C12N 15/10, C12N 15/11, C12Q 1/6844, Patent No. EP1320630, Priority date 29 Aug 2000, Priority No. AUPQ974900
Research output: Patent

Cite this

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title = "Degenerate oligonucleotide gene shuffling",

abstract = "A method for gene shuffling to form a mutant or chimeric gene, the method conmprising: (a) assigning one or more segments of one or more genes based on regions of encoded amino acid sequence; (b) amplifying the one or more assigned segments of the gene using primers specific for each segment; and (c) causing recombination of the one or more amplified segments to form a mutant or chimeric gene. An oligonucleotide primer suitable for use in gene shuffling, the primer having a non-degenerate core based on a segment or template of a gene to be amplified, and the core being flanked by both 5' and 3' degenerate ends.",

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N2 - A method for gene shuffling to form a mutant or chimeric gene, the method conmprising: (a) assigning one or more segments of one or more genes based on regions of encoded amino acid sequence; (b) amplifying the one or more assigned segments of the gene using primers specific for each segment; and (c) causing recombination of the one or more amplified segments to form a mutant or chimeric gene. An oligonucleotide primer suitable for use in gene shuffling, the primer having a non-degenerate core based on a segment or template of a gene to be amplified, and the core being flanked by both 5' and 3' degenerate ends.

AB - A method for gene shuffling to form a mutant or chimeric gene, the method conmprising: (a) assigning one or more segments of one or more genes based on regions of encoded amino acid sequence; (b) amplifying the one or more assigned segments of the gene using primers specific for each segment; and (c) causing recombination of the one or more amplified segments to form a mutant or chimeric gene. An oligonucleotide primer suitable for use in gene shuffling, the primer having a non-degenerate core based on a segment or template of a gene to be amplified, and the core being flanked by both 5' and 3' degenerate ends.

M3 - Patent

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Y2 - 2001/08/29

ER -

Degenerate oligonucleotide gene shuffling

Abstract

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