Degenerate oligonucleotide gene shuffling

Moreland David Gibbs (Inventor), Peter Leonard Bergquist (Inventor), Kaisu Milja Helena Nevalainen (Inventor)

Research output: Patent

Abstract

A method for gene shuffling to form a mutant or chimeric gene, the method conmprising: (a) assigning one or more segments of one or more genes based on regions of encoded amino acid sequence; (b) amplifying the one or more assigned segments of the gene using primers specific for each segment; and (c) causing recombination of the one or more amplified segments to form a mutant or chimeric gene. An oligonucleotide primer suitable for use in gene shuffling, the primer having a non-degenerate core based on a segment or template of a gene to be amplified, and the core being flanked by both 5' and 3' degenerate ends.
Original languageEnglish
Patent numberWO2002018629
Publication statusPublished - 2004

Bibliographical note

Patent owner: Macquarie Research Ltd

Fingerprint Dive into the research topics of 'Degenerate oligonucleotide gene shuffling'. Together they form a unique fingerprint.

  • Cite this

    Gibbs, M. D., Bergquist, P. L., & Nevalainen, K. M. H. (2004). Degenerate oligonucleotide gene shuffling. (Patent No. WO2002018629).