Degenerate oligonucleotide gene shuffling

Moreland David Gibbs (Inventor), Peter Leonard Bergquist (Inventor), Helena Kaisu Milja Nevalainen (Inventor)

Research output: Patent

Abstract

A method for gene shuffling to form a mutant or chimeric gene, the method comprising: (a) assigning one or more segments of one or more genes based on regions of encoded amino acid sequence; (b) amplifying the one or more assigned segments of the gene using primers specific for each segment; and (c) causing recombination of the one or more amplified segments to form a mutant or chimeric gene. An oligonucleotide primer suitable for use in gene shuffling, the primer having a non-degenerate core based on a segment or template of a gene to be amplified, and the core being flanked by both 5' and 3' degenerate ends.

Original languageEnglish
Patent numberUS20040053267
IPCC12N 15/09,C12P 19/00,C12P 19/34,C07H 21/00,C07H 21/04,C12N 15/10,C12N 15/11,C12Q 1/68
Priority date29/08/00
Publication statusPublished - 10 Aug 2010

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