Degenerate oligonucleotide gene shuffling

Moreland David Gibbs; Peter Leonard Bergquist; Helena Kaisu Milja Nevalainen

Degenerate oligonucleotide gene shuffling

Moreland David Gibbs (Inventor), Peter Leonard Bergquist (Inventor), Helena Kaisu Milja Nevalainen (Inventor)

Macquarie University

Research output: Patent

Abstract

A method for gene shuffling to form a mutant or chimeric gene, the method comprising: (a) assigning one or more segments of one or more genes based on regions of encoded amino acid sequence; (b) amplifying the one or more assigned segments of the gene using primers specific for each segment; and (c) causing recombination of the one or more amplified segments to form a mutant or chimeric gene. An oligonucleotide primer suitable for use in gene shuffling, the primer having a non-degenerate core based on a segment or template of a gene to be amplified, and the core being flanked by both 5' and 3' degenerate ends.

Original language	English
Patent number	US20040053267
IPC	C12N 15/09,C12P 19/00,C12P 19/34,C07H 21/00,C07H 21/04,C12N 15/10,C12N 15/11,C12Q 1/68
Priority date	29/08/00
Publication status	Published - 10 Aug 2010

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Degenerate oligonucleotide gene shuffling
Bergquist, P. L. (Inventor), Gibbs, M. D. (Inventor) & Nevalainen, H. K. M. (Inventor), 4 Nov 2004, IPC No. C12N 15/09, C12N 15/10, C12N 15/11, C12Q 1/68, Patent No. AU2001283710, Priority date 29 Aug 2000, Priority No. AUPQ9749
Research output: Patent

Open Access
Degenerate oligonucleotide gene shuffling
Gibbs, M. D. (Inventor), Bergquist, P. L. (Inventor) & Nevalainen, H. K. M. (Inventor), 25 Jun 2003, (Submitted) IPC No. C12N 15/09, C12Q 1/68, C12N 15/10, C12N 15/11, C12Q 1/6844, Patent No. EP1320630, Priority date 29 Aug 2000, Priority No. AUPQ974900
Research output: Patent

Cite this

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title = "Degenerate oligonucleotide gene shuffling",

abstract = "A method for gene shuffling to form a mutant or chimeric gene, the method comprising: (a) assigning one or more segments of one or more genes based on regions of encoded amino acid sequence; (b) amplifying the one or more assigned segments of the gene using primers specific for each segment; and (c) causing recombination of the one or more amplified segments to form a mutant or chimeric gene. An oligonucleotide primer suitable for use in gene shuffling, the primer having a non-degenerate core based on a segment or template of a gene to be amplified, and the core being flanked by both 5' and 3' degenerate ends.",

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N2 - A method for gene shuffling to form a mutant or chimeric gene, the method comprising: (a) assigning one or more segments of one or more genes based on regions of encoded amino acid sequence; (b) amplifying the one or more assigned segments of the gene using primers specific for each segment; and (c) causing recombination of the one or more amplified segments to form a mutant or chimeric gene. An oligonucleotide primer suitable for use in gene shuffling, the primer having a non-degenerate core based on a segment or template of a gene to be amplified, and the core being flanked by both 5' and 3' degenerate ends.

AB - A method for gene shuffling to form a mutant or chimeric gene, the method comprising: (a) assigning one or more segments of one or more genes based on regions of encoded amino acid sequence; (b) amplifying the one or more assigned segments of the gene using primers specific for each segment; and (c) causing recombination of the one or more amplified segments to form a mutant or chimeric gene. An oligonucleotide primer suitable for use in gene shuffling, the primer having a non-degenerate core based on a segment or template of a gene to be amplified, and the core being flanked by both 5' and 3' degenerate ends.

M3 - Patent

M1 - US20040053267

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Degenerate oligonucleotide gene shuffling

Abstract

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