Abstract
A method for gene shuffling to form a mutant or chimeric gene, the method conmprising: (a) assigning one or more segments of one or more genes based on regions of encoded amino acid sequence; (b) amplifying the one or more assigned segments of the gene using primers specific for each segment; and (c) causing recombination of the one or more amplified segments to form a mutant or chimeric gene. An oligonucleotide primer suitable for use in gene shuffling, the primer having a non-degenerate core based on a segment or template of a gene to be amplified, and the core being flanked by both 5' and 3' degenerate ends.
| Original language | English |
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| Patent number | EP1320630 |
| IPC | C12N 15/09,C12Q 1/68,C12N 15/10,C12N 15/11,C12Q 1/6844 |
| Priority date | 29/08/00 |
| Publication status | Submitted - 25 Jun 2003 |