@misc{1a6f7d04a9874172a3039ea81262ac50,
title = "Degenerate oligonucleotide gene shuffling",
abstract = "A method for gene shuffling to form a mutant or chimeric gene, the method conmprising: (a) assigning one or more segments of one or more genes based on regions of encoded amino acid sequence; (b) amplifying the one or more assigned segments of the gene using primers specific for each segment; and (c) causing recombination of the one or more amplified segments to form a mutant or chimeric gene. An oligonucleotide primer suitable for use in gene shuffling, the primer having a non-degenerate core based on a segment or template of a gene to be amplified, and the core being flanked by both 5' and 3' degenerate ends.",
author = "Gibbs, {Moreland David} and Bergquist, {Peter Leonard} and Nevalainen, {Helena Kaisu Milja}",
year = "2003",
month = jun,
day = "25",
language = "English",
type = "Patent",
note = "EP1320630; C12N 15/09,C12Q 1/68,C12N 15/10,C12N 15/11,C12Q 1/6844",
}