Deiodinase type II and tissue specific mRNA alternative splicing in the Australian lungfish, Neoceratodus forsteri

Margareta Sutija*, Terrence J. Longhurst, J. M P Joss

*Corresponding author for this work

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Deiodinase type II metabolises the prohormone T4 (thyroxine) into the biologically active hormone T3 (3,5,3-triiodothyronine), at the cellular level in extrathyroidal target tissues. In juvenile lungfish, Neoceratodus forsteri, a typical deiodinase type II is present in most tissues. We have identified the full length of a 1.8kb deiodinase type II mRNA in liver, and a truncated (1.3kb) version in brain. Both mRNAs have two in frame UGA codons, but only the liver form has a predicted SECIS structure (form 1) in its 3-UTR. We also report the presence of additional different length transcripts of deiodinase II mRNA, i.e., 3, 4, and 8kb, in liver, and 8kb in kidney, heart, and gill tissues. Expression of the longer (∼8kb) transcript is very low. Real-time PCR confirmed the low expression of transcripts in all tissues, suggested by the Northern blot analysis.

Original languageEnglish
Pages (from-to)409-417
Number of pages9
JournalGeneral and Comparative Endocrinology
Volume132
Issue number3
DOIs
Publication statusPublished - 1 Jul 2003

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