Deiodinase type III in the Australian lungfish, Neoceratodus forsteri

Margareta Sutija*, Terrence J. Longhurst, J. M P Joss

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    14 Citations (Scopus)


    This work presents characterisation of deiodinase type III (D3) mRNA as cDNA and the tissue distribution of D3 mRNA in the Australian lungfish, Neoceratodus forsteri. We have identified the full length of a ∼1.4kb D3 mRNA in the liver, which has a single in-frame UGA codon and a selenocysteine insertion sequence (SECIS) form 2 in the 3′-UTR. Lungfish D3 mRNA was expressed in all tested tissues (liver, lung, kidney, brain, heart, and gills) as demonstrated by Northern blot analyses. PCR conducted on genomic DNA indicated that the lungfish D3 is a single exon gene. Also, we present enzymatic characteristics of this exclusively IRD enzyme, have determined its substrate preference, DTT cofactor requirements, PTU inhibition, and kinetic properties. These results indicate that lungfish D3 has the typical enzymatic characteristics of vertebrate D3 enzymes.

    Original languageEnglish
    Pages (from-to)152-161
    Number of pages10
    JournalGeneral and Comparative Endocrinology
    Issue number2
    Publication statusPublished - Apr 2004


    Dive into the research topics of 'Deiodinase type III in the Australian lungfish, Neoceratodus forsteri'. Together they form a unique fingerprint.

    Cite this