Deiodinase type III in the Australian lungfish, Neoceratodus forsteri

Margareta Sutija; Terrence J. Longhurst; J. M P Joss

doi:10.1016/j.ygcen.2003.12.013

Deiodinase type III in the Australian lungfish, Neoceratodus forsteri

Margareta Sutija^*, Terrence J. Longhurst, J. M P Joss

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

This work presents characterisation of deiodinase type III (D3) mRNA as cDNA and the tissue distribution of D3 mRNA in the Australian lungfish, Neoceratodus forsteri. We have identified the full length of a ∼1.4kb D3 mRNA in the liver, which has a single in-frame UGA codon and a selenocysteine insertion sequence (SECIS) form 2 in the 3′-UTR. Lungfish D3 mRNA was expressed in all tested tissues (liver, lung, kidney, brain, heart, and gills) as demonstrated by Northern blot analyses. PCR conducted on genomic DNA indicated that the lungfish D3 is a single exon gene. Also, we present enzymatic characteristics of this exclusively IRD enzyme, have determined its substrate preference, DTT cofactor requirements, PTU inhibition, and kinetic properties. These results indicate that lungfish D3 has the typical enzymatic characteristics of vertebrate D3 enzymes.

Original language	English
Pages (from-to)	152-161
Number of pages	10
Journal	General and Comparative Endocrinology
Volume	136
Issue number	2
DOIs	https://doi.org/10.1016/j.ygcen.2003.12.013
Publication status	Published - Apr 2004

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title = "Deiodinase type III in the Australian lungfish, Neoceratodus forsteri",

abstract = "This work presents characterisation of deiodinase type III (D3) mRNA as cDNA and the tissue distribution of D3 mRNA in the Australian lungfish, Neoceratodus forsteri. We have identified the full length of a ∼1.4kb D3 mRNA in the liver, which has a single in-frame UGA codon and a selenocysteine insertion sequence (SECIS) form 2 in the 3′-UTR. Lungfish D3 mRNA was expressed in all tested tissues (liver, lung, kidney, brain, heart, and gills) as demonstrated by Northern blot analyses. PCR conducted on genomic DNA indicated that the lungfish D3 is a single exon gene. Also, we present enzymatic characteristics of this exclusively IRD enzyme, have determined its substrate preference, DTT cofactor requirements, PTU inhibition, and kinetic properties. These results indicate that lungfish D3 has the typical enzymatic characteristics of vertebrate D3 enzymes.",

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AB - This work presents characterisation of deiodinase type III (D3) mRNA as cDNA and the tissue distribution of D3 mRNA in the Australian lungfish, Neoceratodus forsteri. We have identified the full length of a ∼1.4kb D3 mRNA in the liver, which has a single in-frame UGA codon and a selenocysteine insertion sequence (SECIS) form 2 in the 3′-UTR. Lungfish D3 mRNA was expressed in all tested tissues (liver, lung, kidney, brain, heart, and gills) as demonstrated by Northern blot analyses. PCR conducted on genomic DNA indicated that the lungfish D3 is a single exon gene. Also, we present enzymatic characteristics of this exclusively IRD enzyme, have determined its substrate preference, DTT cofactor requirements, PTU inhibition, and kinetic properties. These results indicate that lungfish D3 has the typical enzymatic characteristics of vertebrate D3 enzymes.

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Deiodinase type III in the Australian lungfish, Neoceratodus forsteri

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