Deletion of the actin-associated tropomyosin Tpm3 leads to reduced cell complexity in cultured hippocampal neurons: new insights into the role of the C-terminal region of Tpm3.1

Tamara Tomanic, Claire Martin, Holly Stefen, Esmeralda Paric, Peter Gunning, Thomas Fath*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)
12 Downloads (Pure)

Abstract

Tropomyosins (Tpms) have been described as master regulators of actin, with Tpm3 products shown to be involved in early developmental processes, and the Tpm3 isoform Tpm3.1 controlling changes in the size of neuronal growth cones and neurite growth. Here, we used primary mouse hippocampal neurons of C57/Bl6 wild type and Bl6Tpm3flox transgenic mice to carry out morphometric analyses in response to the absence of Tpm3 products, as well as to investigate the effect of C-terminal truncation on the ability of Tpm3.1 to modulate neuronal morphogenesis. We found that the knock-out of Tpm3 leads to decreased neurite length and complexity, and that the deletion of two amino acid residues at the C-terminus of Tpm3.1 leads to more detrimental changes in neurite morphology than the deletion of six amino acid residues. We also found that Tpm3.1 that lacks the 6 C-terminal amino acid residues does not associate with stress fibres, does not segregate to the tips of neurites, and does not impact the amount of the filamentous actin pool at the axonal growth cones, as opposed to Tpm3.1, which lacks the two C-terminal amino acid residues. Our study provides further insight into the role of both Tpm3 products and the C-terminus of Tpm3.1, and it forms the basis for future studies that aim to identify the molecular mechanisms underlying Tpm3.1 targeting to different subcellular compartments.
Original languageEnglish
Article number715
Pages (from-to)1-21
Number of pages21
JournalCells
Volume10
Issue number3
DOIs
Publication statusPublished - 23 Mar 2021

Bibliographical note

Copyright the Author(s) 2021. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

Keywords

  • neurite growth
  • actin cytoskeleton
  • protein segregation

Fingerprint

Dive into the research topics of 'Deletion of the actin-associated tropomyosin Tpm3 leads to reduced cell complexity in cultured hippocampal neurons: new insights into the role of the C-terminal region of Tpm3.1'. Together they form a unique fingerprint.

Cite this