Delivery of differentiation factors by mesoporous silica particles assists advanced differentiation of transplanted murine embryonic stem cells

Alfonso E. Garcia-Bennett, Mariya Kozhevnikova, Niclas König, Chunfang Zhou, Richardson Leao, Thomas Knöpfel, Stanislava Pankratova, Carl Trolle, Vladimir Berezin, Elisabeth Bock, Håkan Aldskogius, Elena N. Kozlova

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)

Abstract

Stem cell transplantation holds great hope for the replacement of damaged cells in the nervous system. However, poor long-term survival after transplantation and insufficiently robust differentiation of stem cells into specialized cell types in vivo remain major obstacles for clinical application. Here, we report the development of a novel technological approach for the local delivery of exogenous trophic factor mimetics to transplanted cells using specifically designed silica nanoporous particles. We demonstrated that delivering Cintrofin and Gliafin, established peptide mimetics of the ciliary neurotrophic factor and glial cell line-derived neurotrophic factor, respectively, with these particles enabled not only robust functional differentiation of motor neurons from transplanted embryonic stem cells but also their long-term survival in vivo. We propose that the delivery of growth factors by mesoporous nanoparticles is a potentially versatile and widely applicable strategy for efficient differentiation and functional integration of stem cell derivatives upon transplantation.

Original languageEnglish
Pages (from-to)906-915
Number of pages10
JournalStem Cells Translational Medicine
Volume2
Issue number11
DOIs
Publication statusPublished - 2013
Externally publishedYes

Keywords

  • Cell transplantation
  • Differentiation
  • Embryonic stem cells
  • Nervous system
  • Neural differentiation
  • Neural stem cell
  • Stem cell culture
  • Transplantation

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