Delivery of pDNA to alveolar epithelial cells via nebulization

Larissa G. Reis, Maree Svolos, Lyn M. Moir, Rima Jaber, Norbert Windhab, Paul M. Young, Daniela Traini

Research output: Chapter in Book/Report/Conference proceedingConference proceeding contributionpeer-review

Abstract

Gene therapies are still limited by the current inability to deliver nucleic acids into the cells, as well as intracellular degradation and plasmid deoxyribonucleic acid (pDNA) entrapment in endocytic vesicles. Specifically, for lung delivery, maintenance of the DNA supercoiled structure during aerosolization is very challenging, due to the shear forces experienced as nebulizers (air jet, ultrasonic, mesh) generate droplets. The aim of this study was to investigate the delivery of pDNA into lung alveolar cells using a mesh nebulizer.
Original languageEnglish
Title of host publicationRDD Europe 2017
EditorsR. N. Dalby, J. Peart, J. D. Suman, P. M. Young, D. Traini
Place of PublicationRichmod, VA
PublisherRDD Online
Pages405-410
Number of pages6
Volume2
ISBN (Electronic)9781942911067
Publication statusPublished - 2017
Externally publishedYes
EventRespiratory Drug Delivery Europe 2017 - Antibes, France
Duration: 25 Apr 201728 Apr 2017

Conference

ConferenceRespiratory Drug Delivery Europe 2017
CountryFrance
CityAntibes
Period25/04/1728/04/17

Keywords

  • A549 cells
  • cellular uptake
  • gene delivery
  • lysosome
  • mesh nebulizer

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