Delivery of pDNA to alveolar epithelial cells via nebulization

Larissa G. Reis; Maree Svolos; Lyn M. Moir; Rima Jaber; Norbert Windhab; Paul M. Young; Daniela Traini

Delivery of pDNA to alveolar epithelial cells via nebulization

Larissa G. Reis, Maree Svolos, Lyn M. Moir, Rima Jaber, Norbert Windhab, Paul M. Young, Daniela Traini

Research output: Chapter in Book/Report/Conference proceeding › Conference proceeding contribution › peer-review

Abstract

Gene therapies are still limited by the current inability to deliver nucleic acids into the cells, as well as intracellular degradation and plasmid deoxyribonucleic acid (pDNA) entrapment in endocytic vesicles. Specifically, for lung delivery, maintenance of the DNA supercoiled structure during aerosolization is very challenging, due to the shear forces experienced as nebulizers (air jet, ultrasonic, mesh) generate droplets. The aim of this study was to investigate the delivery of pDNA into lung alveolar cells using a mesh nebulizer.

Original language	English
Title of host publication	RDD Europe 2017
Editors	R. N. Dalby, J. Peart, J. D. Suman, P. M. Young, D. Traini
Place of Publication	Richmod, VA
Publisher	RDD Online
Pages	405-410
Number of pages	6
Volume	2
ISBN (Electronic)	9781942911067
Publication status	Published - 2017
Externally published	Yes
Event	Respiratory Drug Delivery Europe 2017 - Antibes, France Duration: 25 Apr 2017 → 28 Apr 2017

Conference

Conference	Respiratory Drug Delivery Europe 2017
Country/Territory	France
City	Antibes
Period	25/04/17 → 28/04/17

Keywords

A549 cells
cellular uptake
gene delivery
lysosome
mesh nebulizer

Access to Document

https://www.rddonline.com/rdd/article.php?id=0&sid=103&ArticleID=2347&return=1

Cite this

@inproceedings{34f4f97d1e2b4d329e4ca4652da6ac1b,

title = "Delivery of pDNA to alveolar epithelial cells via nebulization",

abstract = "Gene therapies are still limited by the current inability to deliver nucleic acids into the cells, as well as intracellular degradation and plasmid deoxyribonucleic acid (pDNA) entrapment in endocytic vesicles. Specifically, for lung delivery, maintenance of the DNA supercoiled structure during aerosolization is very challenging, due to the shear forces experienced as nebulizers (air jet, ultrasonic, mesh) generate droplets. The aim of this study was to investigate the delivery of pDNA into lung alveolar cells using a mesh nebulizer.",

keywords = "A549 cells, cellular uptake, gene delivery, lysosome, mesh nebulizer",

author = "{G. Reis}, Larissa and Maree Svolos and Moir, {Lyn M.} and Rima Jaber and Norbert Windhab and Young, {Paul M.} and Daniela Traini",

year = "2017",

language = "English",

volume = "2",

pages = "405--410",

editor = "Dalby, {R. N.} and J. Peart and Suman, {J. D.} and Young, {P. M.} and D. Traini",

booktitle = "RDD Europe 2017",

publisher = "RDD Online",

note = "Respiratory Drug Delivery Europe 2017 ; Conference date: 25-04-2017 Through 28-04-2017",

}

G. Reis, L, Svolos, M, Moir, LM, Jaber, R, Windhab, N, Young, PM & Traini, D 2017, Delivery of pDNA to alveolar epithelial cells via nebulization. in RN Dalby, J Peart, JD Suman, PM Young & D Traini (eds), RDD Europe 2017. vol. 2, RDD Online, Richmod, VA, pp. 405-410, Respiratory Drug Delivery Europe 2017, Antibes, France, 25/04/17. <https://www.rddonline.com/rdd/article.php?id=0&sid=103&ArticleID=2347&return=1>

TY - GEN

T1 - Delivery of pDNA to alveolar epithelial cells via nebulization

AU - G. Reis, Larissa

AU - Svolos, Maree

AU - Moir, Lyn M.

AU - Jaber, Rima

AU - Windhab, Norbert

AU - Young, Paul M.

AU - Traini, Daniela

PY - 2017

Y1 - 2017

N2 - Gene therapies are still limited by the current inability to deliver nucleic acids into the cells, as well as intracellular degradation and plasmid deoxyribonucleic acid (pDNA) entrapment in endocytic vesicles. Specifically, for lung delivery, maintenance of the DNA supercoiled structure during aerosolization is very challenging, due to the shear forces experienced as nebulizers (air jet, ultrasonic, mesh) generate droplets. The aim of this study was to investigate the delivery of pDNA into lung alveolar cells using a mesh nebulizer.

AB - Gene therapies are still limited by the current inability to deliver nucleic acids into the cells, as well as intracellular degradation and plasmid deoxyribonucleic acid (pDNA) entrapment in endocytic vesicles. Specifically, for lung delivery, maintenance of the DNA supercoiled structure during aerosolization is very challenging, due to the shear forces experienced as nebulizers (air jet, ultrasonic, mesh) generate droplets. The aim of this study was to investigate the delivery of pDNA into lung alveolar cells using a mesh nebulizer.

KW - A549 cells

KW - cellular uptake

KW - gene delivery

KW - lysosome

KW - mesh nebulizer

M3 - Conference proceeding contribution

VL - 2

SP - 405

EP - 410

BT - RDD Europe 2017

A2 - Dalby, R. N.

A2 - Peart, J.

A2 - Suman, J. D.

A2 - Young, P. M.

A2 - Traini, D.

PB - RDD Online

CY - Richmod, VA

T2 - Respiratory Drug Delivery Europe 2017

Y2 - 25 April 2017 through 28 April 2017

ER -

Delivery of pDNA to alveolar epithelial cells via nebulization

Abstract

Conference

Keywords

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